Tran Phu-Tri, Widyasari Kristin, Seo Jang-Kyun, Kim Kook-Hyung
Department of Agricultural Biotechnology and College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Department of Agricultural Biotechnology and College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Virology. 2018 Jan 1;513:153-159. doi: 10.1016/j.virol.2017.10.014. Epub 2017 Nov 5.
Soybean mosaic virus (SMV), a member of the genus Potyvirus, significantly reduces soybean production worldwide. Rsv3, which confers strain-specific resistance to SMV, was previously mapped between the markers A519F/R and M3Satt in chromosome 14 of the soybean [Glycine max (L.) Merr.] genotype L29. Analysis of the soybean genome database revealed that five different NBS-LRR sequences exist between the flanking markers. Among these candidate Rsv3 genes, the full-length cDNA of the Glyma.14g204700 was successfully cloned from L29. Over-expression of Glyma.14g204700 in leaves inoculated with SMV inhibited viral infection in a soybean genotype lacking Rsv3. In addition, the transient silencing of the candidate gene caused a high accumulation of an avirulent strain in L29 carrying Rsv3. Our results therefore provide additional line of evidence to support that Glyma.14g204700 is likely Rsv3 gene that confers strain-specific resistance to SMV.
大豆花叶病毒（SMV）是马铃薯Y病毒属的成员之一，在全球范围内显著降低大豆产量。Rsv3基因赋予对SMV的株系特异性抗性，此前已定位在大豆[Glycine max (L.) Merr.]基因型L29的第14号染色体上的标记A519F/R和M3Satt之间。对大豆基因组数据库的分析表明，侧翼标记之间存在五个不同的NBS-LRR序列。在这些候选Rsv3基因中，成功从L29中克隆出Glyma.14g204700的全长cDNA。在接种SMV的叶片中过表达Glyma.14g204700可抑制缺乏Rsv3的大豆基因型中的病毒感染。此外，候选基因的瞬时沉默导致携带Rsv3的L29中无毒株系的高积累。因此，我们的结果提供了额外的证据支持Glyma.14g204700可能是赋予对SMV株系特异性抗性的Rsv3基因。
