Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, 813 72 Bratislava, Slovakia.
Faculty of Public Health, Department of Toxicology, Slovak Medical University, Limbová 12, 833 03 Bratislava, Slovakia.
Oxid Med Cell Longev. 2017;2017:7430435. doi: 10.1155/2017/7430435. Epub 2017 Sep 10.
Our goal was to evaluate the potential health risk of the polymeric NP, poly(ethylene glycol)--poly(lactic acid) (PEG--PLA), from the view of redox imbalance of the organism in two different life stages. Female Wistar rats were neonatally administered intraperitoneally with PEG--PLA NPs [20 mg/kg of b.w. (PEG20) or 40 (PEG40) mg/kg of b.w.] from postnatal day 4 (PND4) to PND7. We measured antioxidant capacity (TEAC), level of protein carbonyls and lipoperoxides in plasma, activities of catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD) in hemolysates of infantile (sacrificed on PND17) and adult (sacrificed after PND176) rats. Compared to controls, neonatal PEG40 exposure induced a significant TEAC reduction in the infantile rats. Protein carbonyls and lipoperoxide levels were not affected after any dose of PEG--PLA NP administration. In adult rats, PEG20 administration caused a significant decrease of protein carbonyl levels compared to controls. In infantile rats, both doses of PEG--PLA NP administration increased catalase, Gpx, and SOD activities compared to controls. Surprisingly, in adult rats, the activities of Gpx and SOD decreased significantly after administration of both doses of PEG--PLA NPs. Obtained data indicate a possible age-related association between the oxidative status and neonatal PEG--PLA NP administration in female rats.
我们的目标是从机体氧化失衡的角度评估聚合纳米颗粒（poly(ethylene glycol)--poly(lactic acid)，PEG--PLA）在两个不同生命阶段的潜在健康风险。雌性 Wistar 大鼠从出生后第 4 天（PND4）到第 7 天（PND7）腹膜内给予 PEG--PLA 纳米颗粒[20mg/kg 体重（PEG20）或 40mg/kg 体重（PEG40）]。我们测量了幼鼠（于 PND17 处死）和成年鼠（PND176 后处死）血浆中的抗氧化能力（TEAC）、蛋白羰基和脂质过氧化物水平、血红素中过氧化氢酶、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的活性。与对照组相比，新生期 PEG40 暴露使幼鼠的 TEAC 显著降低。任何剂量的 PEG--PLA NP 给药后，蛋白羰基和脂质过氧化物水平均不受影响。在成年大鼠中，PEG20 给药与对照组相比，蛋白羰基水平显著降低。在幼鼠中，PEG--PLA NP 的两种剂量给药均使过氧化氢酶、GPx 和 SOD 活性与对照组相比显著升高。令人惊讶的是，在成年大鼠中，两种剂量的 PEG--PLA NP 给药后，GPx 和 SOD 的活性均显著降低。这些数据表明，在雌性大鼠中，氧化状态与新生期 PEG--PLA NP 给药之间可能存在年龄相关的关联。
