Laubertova Lucia, Dvorakova Monika, Balis Peter, Puzserova Angelika, Zitnanova Ingrid, Bernatova Iveta
Faculty of Medicine, Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, Sasinkova 2, 813 72 Bratislava, Slovakia.
Institute of Normal and Pathological Physiology, Centre of Experimental Medicine, Slovak Academy of Sciences, v.v.i., 813 71 Bratislava, Slovakia.
Antioxidants (Basel). 2022 Apr 9;11(4):751. doi: 10.3390/antiox11040751.
Several studies have reported that the administration of various nanoparticles in vivo can cause oxidative stress. The combination of ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) and acute stress was selected because, during intravenous application of a contrast agent, patients are exposed to psycho-emotional stress. This study was designed to investigate the effect of acute stress and USPIONs on selected markers of oxidative stress (antioxidant capacity, superoxide dismutase, glutathione peroxidase and catalase activities, levels of advanced oxidation protein products, protein carbonyls, lipoperoxides and 8-isoprostanes) in plasma and erythrocytes in normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). In the WKY and SHR groups, there was a significant main effect of genotype between groups on studied markers except protein carbonyls and lipoperoxides. In SHR, the combination of acute stress and USPIONs increased the antioxidant capacity of plasma and the selected enzyme activities of erythrocytes. In WKY, the combination of acute stress and USPIONs decreased the antioxidant capacity of erythrocytes and reduced levels of advanced oxidation protein products in plasma. Our study points to the fact that, when hypertensive subjects are treated with iron oxide nanoparticles, caution should be taken, especially in stress conditions, since they seem to be more vulnerable to oxidative stress produced by USPIONs.
多项研究报告称，在体内施用各种纳米颗粒可导致氧化应激。选择超小超顺磁性氧化铁纳米颗粒（USPIONs）与急性应激相结合，是因为在静脉注射造影剂期间，患者会面临心理情绪压力。本研究旨在调查急性应激和USPIONs对正常血压的Wistar-Kyoto大鼠（WKY）和自发性高血压大鼠（SHR）血浆和红细胞中氧化应激的选定标志物（抗氧化能力、超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性、晚期氧化蛋白产物水平、蛋白质羰基、脂过氧化物和8-异前列腺素）的影响。在WKY和SHR组中，除蛋白质羰基和脂过氧化物外，各基因型组之间对研究标志物存在显著的主要效应。在SHR中，急性应激和USPIONs的组合增加了血浆的抗氧化能力和红细胞的选定酶活性。在WKY中，急性应激和USPIONs的组合降低了红细胞的抗氧化能力，并降低了血浆中晚期氧化蛋白产物的水平。我们的研究指出，当高血压患者用氧化铁纳米颗粒治疗时，应谨慎行事，尤其是在应激条件下，因为他们似乎更容易受到USPIONs产生的氧化应激的影响。
