Lördal M, Hellström P M, Johansson C
Dept. of Internal Medicine, Karolinska Hospital, Stockholm, Sweden.
Scand J Gastroenterol Suppl. 1988;152:48-53. doi: 10.3109/00365528809095933.
The aim of the present study was to investigate the effect of cisapride on the myoelectric activity of the small intestine in the rat. Intestinal myoelectric activity of fasted conscious rats was monitored by three bipolar electrodes chronically implanted at 5, 15 and 25 cm distal to the pylorus and connected to an EEG amplifier. In the basal state, regularly occurring migrating myoelectric complexes (MMCs) and clustered spikes were registered. Cisapride at doses of 0.5-4.0 mg-1 i.v. did not affect the MMCs, but at 8 mg kg-1 i.v. the regular MMCs were replaced by irregular spiking activity (p less than 0.05). Concomitantly cisapride increased the occurrence of clustered spikes and abbreviated the interval between them in a dose-dependent manner (p less than 0.05), without affecting their duration. Hexamethonium at a dose of 10 mg kg-1 i.v. abolished the MMCs as well as the stimulatory effect of ciSapride (4 mg kg-1). Atropine at a dose of 1 mg kg-1 i.v. did not affect the MMCs but blocked the stimulatory effect of cisapride (4 mg kg-1). It is concluded that cisapride induces clustered spikes and irregular spiking. These effects require intact cholinergic pathways, involving both muscarinic and nicotinic receptor mechanisms.
本研究的目的是探讨西沙必利对大鼠小肠肌电活动的影响。通过将三个双极电极长期植入幽门远端5、15和25厘米处，并连接到脑电图放大器，监测禁食清醒大鼠的肠道肌电活动。在基础状态下，记录到有规律出现的移行性肌电复合波（MMCs）和簇状棘波。静脉注射剂量为0.5 - 4.0毫克/千克的西沙必利对MMCs无影响，但静脉注射8毫克/千克时，规则的MMCs被不规则的棘波活动所取代（p小于0.05）。同时，西沙必利以剂量依赖性方式增加了簇状棘波的发生率并缩短了它们之间的间隔（p小于0.05），而不影响其持续时间。静脉注射剂量为10毫克/千克的六甲铵消除了MMCs以及西沙必利（4毫克/千克）的刺激作用。静脉注射剂量为1毫克/千克的阿托品不影响MMCs，但阻断了西沙必利（4毫克/千克）的刺激作用。结论是西沙必利诱导簇状棘波和不规则棘波。这些作用需要完整的胆碱能途径，涉及毒蕈碱和烟碱受体机制。
