Xie Jinglin L, Bohovych Iryna, Wong Erin O Y, Lambert Jean-Philippe, Gingras Anne-Claude, Khalimonchuk Oleh, Cowen Leah E, Leach Michelle D
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.
Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA.
Microb Cell. 2017 Oct 2;4(10):342-361. doi: 10.15698/mic2017.10.594.
Mitochondria underpin metabolism, bioenergetics, signalling, development and cell death in eukaryotes. Most of the ~1,000 yeast mitochondrial proteins are encoded in the nucleus and synthesised as precursors in the cytosol, with mitochondrial import facilitated by molecular chaperones. Here, we focus on the Hsp40 chaperone Ydj1 in the fungal pathogen , finding that it is localised to both the cytosol and outer mitochondrial membrane, and is required for cellular stress responses and for filamentation, a key virulence trait. Mapping the Ydj1 protein interaction network highlighted connections with co-chaperones and regulators of filamentation. Furthermore, the mitochondrial processing peptidases Mas1 and Mas2 were highly enriched for interaction with Ydj1. Additional analysis demonstrated that loss of , or perturbs mitochondrial morphology and function. Deletion of impairs import of Su9, a protein that is cleaved to a mature form by Mas1 and Mas2. Thus, we highlight a novel role for Ydj1 in cellular morphogenesis, stress responses, and mitochondrial import in the fungal kingdom.
线粒体是真核生物新陈代谢、生物能量学、信号传导、发育和细胞死亡的基础。酵母中约1000种线粒体蛋白大多由细胞核编码，在细胞质中以前体形式合成，分子伴侣促进其导入线粒体。在这里，我们聚焦于真菌病原体中的热休克蛋白40（Hsp40）伴侣蛋白Ydj1，发现它定位于细胞质和线粒体外膜，是细胞应激反应和丝状化（一种关键的致病性状）所必需的。绘制Ydj1蛋白相互作用网络突出了与伴侣蛋白和丝状化调节因子的联系。此外，线粒体加工肽酶Mas1和Mas2与Ydj1的相互作用高度富集。进一步分析表明，缺失Mas1、Mas2或Ydj1会扰乱线粒体形态和功能。缺失Ydj1会损害Su9的导入，Su9是一种被Mas1和Mas2切割成成熟形式的蛋白。因此，我们突出了Ydj1在真菌王国细胞形态发生、应激反应和线粒体导入中的新作用。
