Genetic Engineering Research Center, School of Life Sciences, Chongqing Universitygrid.190737.b, Chongqing, People's Republic of China.
Chongqing Engineering Research Center for Fungal Insecticide, Chongqing, People's Republic of China.
Microbiol Spectr. 2022 Apr 27;10(2):e0205121. doi: 10.1128/spectrum.02051-21. Epub 2022 Mar 28.
As a conserved sensor kinase in the HOG-MAPK pathway, Sln1 plays distinct functions in different fungi. In this study, the roles of MaSln1 in Metarhizium acridum were analyzed using gene knockout and rescue strategies. Deletion of did not affect conidial germination, conidial yield, or resistance to chemical agents. However, fungal tolerance to heat shock and UV-B were significantly reduced after deletion of . Insect bioassays showed that fungal pathogenicity was significantly impaired when was deleted. Further studies showed that MaSln1 did not affect either germination or appressorium formation of on locust wings, but it significantly increased appressorium turgor pressure. In addition, disruption of resulted in a conidiation pattern shift in . Microscopic observation revealed, however, that some genes located in the MAPK signaling pathway, including , , , and , were not involved in the conidiation pattern shift on SYA medium (microcycle medium). Meanwhile, of the 143 differently expressed genes (DEGs) identified by RNA-seq, no genes related to the MAPK pathway were found, suggesting that MaSln1 regulation of the conidiation pattern shift was probably independent of the conserved MAPK signaling pathway. It was found that 22 of the 98 known DEGs regulated by MaSln1 were involved in mycelial growth, cell division, and cytoskeleton formation, indicating that MaSln1 likely regulates the expression of genes related to cell division and morphogenesis, thus regulating the conidiation pattern shift in . The productivity and quality of conidia are both crucial for mycopesticides. In this study, we systematically analyzed the roles of in fungal pathogens. Most importantly, our results revealed that deletion of resulted in a conidiation pattern shift in . However, some other genes, located in the MAPK signaling pathway, were not involved in the conidiation pattern shift. RNA-seq revealed no genes related to the MAPK pathway, suggesting that the regulation of the conidiation pattern shift by MaSln1 was probably independent of the conserved MAPK signaling pathway. This study provided a new insight into the functions of Sln1 and laid a foundation for exploring the mechanisms of conidiation pattern shifts in .
作为 HOG-MAPK 途径中的一种保守传感器激酶，Sln1 在不同真菌中发挥着不同的功能。在这项研究中，我们使用基因敲除和拯救策略分析了 MaSln1 在绿僵菌中的作用。Δ 突变不影响分生孢子的萌发、分生孢子的产量或对化学试剂的抗性。然而，Δ 突变后真菌对热激和 UV-B 的耐受性显著降低。昆虫生物测定表明，当Δ 时，真菌的致病性显著受损。进一步的研究表明，MaSln1 既不影响分生孢子在蝗虫翅膀上的萌发或附着胞的形成，但它显著增加了附着胞的膨压。此外，Δ 导致产孢模式在 SYA 培养基（微环培养基）上发生转变。然而，显微镜观察显示，MAPK 信号通路中的一些基因，包括 、 、 、 和 ，不参与 SYA 培养基上的产孢模式转变。同时，通过 RNA-seq 鉴定的 143 个差异表达基因（DEGs）中，没有发现与 MAPK 途径相关的基因，这表明 MaSln1 对产孢模式转变的调控可能独立于保守的 MAPK 信号通路。结果发现，MaSln1 调控的 98 个已知 DEGs 中的 22 个与菌丝生长、细胞分裂和细胞骨架形成有关，表明 MaSln1 可能调控与细胞分裂和形态发生有关的基因的表达，从而调控绿僵菌中的产孢模式转变。分生孢子的生产力和质量对真菌杀虫剂都至关重要。在这项研究中，我们系统地分析了 MaSln1 在真菌病原体中的作用。最重要的是，我们的结果表明，Δ 导致产孢模式在 SYA 培养基上发生转变。然而，MAPK 信号通路中的其他一些基因不参与产孢模式转变。RNA-seq 没有发现与 MAPK 途径相关的基因，这表明 MaSln1 对产孢模式转变的调控可能独立于保守的 MAPK 信号通路。本研究为 Sln1 的功能提供了新的见解，并为探索绿僵菌产孢模式转变的机制奠定了基础。
