Center of Excellence for Occupational Health, Occupational Health and Safety Research Center, School of Public health, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Occupational Health Engineering, Faculty of Health, Arak University of Medical Sciences, Arak, Iran.
J Sep Sci. 2018 Jan;41(2):501-508. doi: 10.1002/jssc.201700685. Epub 2017 Nov 27.
In the current study, a novel technique for extraction and determination of trans,trans-muconic acid, hippuric acid, and mandelic acid was developed by means of ion-pair-based hollow fiber liquid-phase microextraction in the three-phase mode. Important factors affecting the extraction efficiency of the method were investigated and optimized. These metabolites were extracted from 10 mL of the source phase into a supported liquid membrane containing 1-octanol and 10% w/v of Aliquat 336 as the ionic carrier followed by high-performance liquid chromatography analysis. The organic phase immobilized in the pores of a hollow fiber was back-extracted into 24 μL of a solution containing 3.0 mol/L sodium chloride placed inside the lumen of the fiber. A very high preconcentration of 212- to 440-fold, limit of detection of 0.1-7 μg/L, and relative recovery of 87-95% were obtained under the optimized conditions of this method. The relative standard deviation values for within-day and between-day precisions were calculated at 2.9-8.5 and 4.3-11.2%, respectively. The method was successfully applied to urine samples from volunteers at different work environments. The results demonstrated that the method can be used as a sensitive and effective technique for the determination of the metabolites in urine.
在当前的研究中，通过三相模式下基于离子对的中空纤维液相微萃取，开发了一种新颖的提取和测定反式，反式-粘康酸、马尿酸和扁桃酸的技术。研究并优化了影响该方法萃取效率的重要因素。这些代谢物从 10 mL 初始相中被提取到含有 1-辛醇和 10%w/v Aliquat 336 的支撑液膜中，随后用高效液相色谱法进行分析。有机相被固定在中空纤维的孔中，然后用含有 3.0 mol/L 氯化钠的 24 μL 溶液从纤维内腔中进行反萃取。在该方法的优化条件下，可实现 212-440 倍的高浓缩，检测限为 0.1-7 μg/L，相对回收率为 87-95%。日内和日间精密度的相对标准偏差值分别为 2.9-8.5%和 4.3-11.2%。该方法成功应用于来自不同工作环境的志愿者的尿液样本。结果表明，该方法可作为尿液中代谢物的灵敏有效测定技术。
