Bahrami Abdulrahman, Ghamari Farhad, Yamini Yadollah, Ghorbani Shahna Farshid, Moghimbeigi Abbas
Excellence Centre of Occupational Health, Research Center for Health Sciences, School of Public Health, Hamadan University of Medical Sciences, Hamadan 6517838736, Iran.
Department of Occupational Health, School of Public Health, Arak University of Medical Sciences, Arak 3819693345, Iran.
Membranes (Basel). 2017 Feb 12;7(1):8. doi: 10.3390/membranes7010008.
This work describes a new extraction method with hollow-fiber liquid-phase microextraction based on facilitated pH gradient transport for analyzing hippuric acid and mandelic acid in aqueous samples. The factors affecting the metabolites extraction were optimized as follows: the volume of sample solution was 10 mL with pH 2 containing 0.5 mol·L sodium chloride, liquid membrane containing 1-octanol with 20% (/) tributyl phosphate as the carrier, the time of extraction was 150 min, and stirring rate was 500 rpm. The organic phase immobilized in the pores of a hollow fiber was back-extracted into 24 µL of a solution containing sodium carbonate with pH 11, which was placed inside the lumen of the fiber. Under optimized conditions, the high enrichment factors of 172 and 195 folds, detection limit of 0.007 and 0.009 µg·mL were obtained. The relative standard deviation (RSD) (%) values for intra- and inter-day precisions were calculated at 2.5%-8.2% and 4.1%-10.7%, respectively. The proposed method was successfully applied to the analysis of these metabolites in real urine samples. The results indicated that hollow-fiber liquid-phase microextraction (HF-LPME) based on facilitated pH gradient transport can be used as a sensitive and effective method for the determination of mandelic acid and hippuric acid in urine specimens.
本研究描述了一种基于促进pH梯度传输的中空纤维液相微萃取新方法，用于分析水性样品中的马尿酸和扁桃酸。影响代谢物萃取的因素优化如下：样品溶液体积为10 mL，pH为2，含有0.5 mol·L氯化钠，液膜为含20%（/）磷酸三丁酯的1-辛醇，萃取时间为150 min，搅拌速率为500 rpm。固定在中空纤维孔中的有机相被反萃取到24 μL pH为11的碳酸钠溶液中，该溶液置于纤维内腔。在优化条件下，获得了172倍和195倍的高富集因子，检测限分别为0.007和0.009 μg·mL。日内和日间精密度的相对标准偏差（RSD）（%）值分别计算为2.5%-8.2%和4.1%-10.7%。该方法成功应用于实际尿液样品中这些代谢物的分析。结果表明，基于促进pH梯度传输的中空纤维液相微萃取（HF-LPME）可作为一种灵敏有效的方法用于尿液标本中扁桃酸和马尿酸的测定。
