Lv Xiaolan, Hou Min, Duan Xiaojing
Department of Ultrasonography, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
Oncol Lett. 2017 Oct;14(4):4641-4646. doi: 10.3892/ol.2017.6785. Epub 2017 Aug 22.
The study aims to investigate the correlation between the parameters of contrast-enhanced ultrasonography in evaluating cervical cancer metastasis and expression of E-cadherin. All 120 patients with cervical cancer underwent contrast-enhanced ultrasonography. According to the results of postoperative pathological examination, patients were divided into distant metastasis group (group A), lymph node metastasis without distant metastasis group (group B) and no metastasis group (group C). Expression of E-cadherin in cervical cancer tissues was detected by enzyme-linked immunosorbent assay (ELISA). Correlations between the parameters of contrast-enhanced ultrasonography in evaluating cervical cancer metastasis and expression of E-cadherin were analyzed by Pearsons correlation analysis. Comparison of parameters of contrast-enhanced ultrasonography showed that, the baseline intensity of group A was 11.9±2.2 dB, which was significantly lower than that of group B and C. Baseline intensity of group B was significantly lower than that of group C (13.0±2.4 vs. 15.3±3.6 dB), significant differences were found among three groups (P<0.05). The sensitivity and specificity of the use of enhanced intensity ~83.7 dB in evaluating tumor metastasis of patients with cervical cancer were 82.42 and 79.32%, respectively. Expression level of E-cadherin protein in group A was 0.030±0.003 ng/ml, which was significantly lower than that in group B and C (P<0.05), expression level of E-cadherin protein in group A was significantly lower than that in group C (0.037±0.007 vs. 0.045±0.012 ng/ml), significant differences in the expression level of E-cadherin protein were found among the three groups of cervical cancer patients (P<0.05). Pearsons correlation analysis showed that there was a positive correlation between the baseline intensity of contrast-enhanced ultrasonography and the expression level of E-cadherin (P<0.05), while there was a negative correlation between the enhanced intensity of contrast-enhanced ultrasonography and the expression level of E-cadherin (P<0.05). Contrast-enhanced ultrasonography can be used to determine the tumor metastasis of cervical cancer patients, in addition, the combined use of contrast-enhanced ultrasonography and E-cadherin protein expression can significantly improve the diagnosis and treatment of cervical cancer.
本研究旨在探讨超声造影评估宫颈癌转移的参数与E-钙黏蛋白表达之间的相关性。120例宫颈癌患者均接受了超声造影检查。根据术后病理检查结果，将患者分为远处转移组（A组）、无远处转移的淋巴结转移组（B组）和无转移组（C组）。采用酶联免疫吸附测定（ELISA）法检测宫颈癌组织中E-钙黏蛋白的表达。采用Pearson相关分析方法分析超声造影评估宫颈癌转移的参数与E-钙黏蛋白表达之间的相关性。超声造影参数比较显示，A组的基线强度为11.9±2.2 dB，显著低于B组和C组。B组的基线强度显著低于C组（13.0±2.4 vs. 15.3±3.6 dB），三组间差异有统计学意义（P<0.05）。采用增强强度≥83.7 dB评估宫颈癌患者肿瘤转移的敏感度和特异度分别为82.42%和79.32%。A组E-钙黏蛋白蛋白表达水平为0.030±0.003 ng/ml，显著低于B组和C组（P<0.05），A组E-钙黏蛋白蛋白表达水平显著低于C组（0.037±0.007 vs. 0.045±0.012 ng/ml），三组宫颈癌患者E-钙黏蛋白蛋白表达水平差异有统计学意义（P<0.05）。Pearson相关分析显示，超声造影基线强度与E-钙黏蛋白表达水平呈正相关（P<0.05），而超声造影增强强度与E-钙黏蛋白表达水平呈负相关（P<0.05）。超声造影可用于判断宫颈癌患者的肿瘤转移情况，此外，联合应用超声造影和E-钙黏蛋白蛋白表达可显著提高宫颈癌的诊断和治疗水平。
