Habibi Parisa, Babri Shirin, Ahmadiasl Nasser, Yousefi Hadi
Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Basic Med Sci. 2017 Aug;20(8):856-862. doi: 10.22038/IJBMS.2017.9106.
The aim of the present study was to investigate the effects of genistein and exercise on the spatial memory and expression of microRNA-132, BDNF, and IGF-1 in the hippocampus of ovariectomized rats.
Sixty animals were divided into six groups of control, sham, ovariectomy (OVX), ovariectomized with 8 weeks of genistein administration (OVX.G), with 8 weeks of swimming training (OVX.E), and with 8 weeks of both of them (OVX.G.E). The effect of genistein and/or exercise was evaluated by measuring microRNA-132, BDNF, and IGF-1 expression levels in the hippocampus tissue. Grafts were analyzed using Real-time polymerase chain reaction for microRNA-132, BDNF, IGF-1, and spatial memory via a Morris water maze (MWM).
Our findings showed that ovariectomy decreased the expression of microRNA-132, BDNF, and IGF-1 in the hippocampus (<0.05) in comparison with the sham group as well as performance in the water maze (<0.05). Also according to results ovariectomized groups that were treated with genistein/exercise or both of them showed significant difference in expression of microRNA-132, BDNF, and IGF-1 in the hippocampus (<0.05) and decreased latency in MWM (<0.05) compared with the OVX group but combination treatment was more effective in the OVX.G.E group in comparison with OVX.E and OVX.G groups.
Overall our results emphasized that combination treatment with genistein and exercise could improve microRNA-132, BDNF, and IGF-1 expression in the hippocampus as well as the spatial memory of ovariectomized rats. These effects may have beneficial impacts on the menopausal period.
本研究旨在探讨染料木黄酮和运动对去卵巢大鼠海马区空间记忆以及微小RNA - 132、脑源性神经营养因子（BDNF）和胰岛素样生长因子-1（IGF - 1）表达的影响。
将60只动物分为六组，即对照组、假手术组、去卵巢组（OVX）、给予8周染料木黄酮的去卵巢组（OVX.G）、进行8周游泳训练的去卵巢组（OVX.E）以及同时进行这两种处理8周的去卵巢组（OVX.G.E）。通过测量海马组织中微小RNA - 132、BDNF和IGF - 1的表达水平来评估染料木黄酮和/或运动的效果。使用实时聚合酶链反应分析移植物中微小RNA - 132、BDNF、IGF - 1，并通过莫里斯水迷宫（MWM）评估空间记忆。
我们的研究结果表明，与假手术组相比，去卵巢降低了海马区微小RNA - 132、BDNF和IGF - 1的表达（<0.05）以及水迷宫中的表现（<0.05）。此外，根据结果，用染料木黄酮/运动或两者处理的去卵巢组与OVX组相比，海马区微小RNA - 132、BDNF和IGF - 1的表达有显著差异（<0.05），并且在MWM中的潜伏期缩短（<0.05），但联合治疗在OVX.G.E组比OVX.E组和OVX.G组更有效。
总体而言，我们的结果强调，染料木黄酮和运动联合治疗可以改善去卵巢大鼠海马区微小RNA - 132、BDNF和IGF - 1的表达以及空间记忆。这些作用可能对绝经期有有益影响。
