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硫喷妥钠作为一种γ-氨基丁酸模拟药物在新生期对新生和成年雄性大鼠海马中谷氨酸脱羧酶65（GAD65）和谷氨酸脱羧酶67（GAD67）基因表达的影响。

The effect of sodium thiopental as a GABA mimetic drug in neonatal period on expression of GAD65 and GAD67 genes in hippocampus of newborn and adult male rats.

作者信息

Naseri Masoud, Parham Abbas, Moghimi Ali

机构信息

Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Embryonic and Stem Cell Biology and Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.

出版信息

Iran J Basic Med Sci. 2017 Sep;20(9):996-1001. doi: 10.22038/IJBMS.2017.9264.

DOI:10.22038/IJBMS.2017.9264
PMID:29085593
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5651466/
Abstract

OBJECTIVES

Development of the nervous system in human and most animals is continued after the birth. Critical role of this period in generation and specialization of the neuronal circuits is confirmed in numerous studies. Any pharmacological intervention in this period may result in structural, functional or behavioral abnormalities. In this study, sodium thiopental a GABA mimetic drug was administrated to newborn rats and their GAD65 and GAD67 expression in hippocampus was evaluated before and after puberty.

MATERIALS AND METHODS

Newborn male Wistar rats were received sodium thiopental (35 mg/kg) daily for 11 days (from 4 to 14 days after birth). Expression of GAD65 and GAD67 in their hippocampus was compared with control groups in 15 and 45 days after birth with RT-qPCR method.

RESULTS

Significant down regulation of GAD65 and GAD67 gene expression was observed in treated rats compared with control group in 45 days after birth animals. But no significant difference was shown between experimental and control groups 15 days after birth animals.

CONCLUSION

The effect of sodium thiopental on GAD65 and GAD67 expression only at adult rats showed a latent period of influence which can be attributed to dosage or intension of sodium thiopental neurotoxicity. Significant down regulation of GAD65 and GAD67 showed unwanted effect of sodium thiopental as GABA mimetic drug in critical period of development.

摘要

目的

人类和大多数动物出生后，神经系统仍在持续发育。众多研究证实了这一时期在神经元回路形成和特化过程中的关键作用。在此期间的任何药物干预都可能导致结构、功能或行为异常。在本研究中，将硫喷妥钠（一种GABA模拟药物）给予新生大鼠，并在青春期前后评估其海马体中GAD65和GAD67的表达。

材料与方法

新生雄性Wistar大鼠连续11天（从出生后第4天至第14天）每天接受硫喷妥钠（35mg/kg）。采用RT-qPCR方法，将其海马体中GAD65和GAD67的表达与出生后15天和45天的对照组进行比较。

结果

与出生后45天的对照组相比，经处理的大鼠中GAD65和GAD67基因表达显著下调。但出生后15天的实验组与对照组之间未显示出显著差异。

结论

硫喷妥钠对GAD65和GAD67表达的影响仅在成年大鼠中表现出潜伏期，这可能归因于硫喷妥钠神经毒性的剂量或强度。GAD65和GAD67的显著下调表明硫喷妥钠作为GABA模拟药物在关键发育时期产生了不良影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3645/5651466/e636019481eb/IJBMS-20-996-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3645/5651466/dfaef2d5bee4/IJBMS-20-996-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3645/5651466/801c31f6b53f/IJBMS-20-996-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3645/5651466/e0e88d3d1019/IJBMS-20-996-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3645/5651466/e636019481eb/IJBMS-20-996-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3645/5651466/dfaef2d5bee4/IJBMS-20-996-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3645/5651466/801c31f6b53f/IJBMS-20-996-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3645/5651466/e0e88d3d1019/IJBMS-20-996-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3645/5651466/e636019481eb/IJBMS-20-996-g004.jpg

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