Gargiulo Sara, Greco Adelaide, Gramanzini Matteo, Esposito Silvia, Affuso Andrea, Brunetti Arturo, Vesce Giancarlo
Bioimaging and Biostructures Institute, Italian National Council of Research, Naples, Italy.
ILAR J. 2012;53(1):E55-69. doi: 10.1093/ilar.53.1.55.
Animal experiments are necessary for a better understanding of diseases and for developing new therapeutic strategies. The mouse (Mus musculus) is currently the most popular laboratory animal in biomedical research. Experimental procedures on animals often require anesthesia and/or analgesia to obtain adequate immobilization and to reduce stress or pain. Mice anesthesia is challenging for several reasons including the animals' size, metabolic rate, and the high risk of hypothermia and hypoglycemia. Moreover, anesthetic agents influence physiological parameters, further interfering with experimental results. Small animal imaging procedures are increasingly used in biomedical research both because the animals allow in vivo monitoring and because they are readily available for longitudinal and noninvasive studies as well as investigations into the evolution of diseases and the effects of new therapies. Anesthesia must adapt to the imaging technique, the procedure length, and the aim of the study. The purpose of this article is to review the existing literature on anesthetic protocols adopted in mice for molecular imaging studies and to report our experience.
动物实验对于更好地理解疾病和开发新的治疗策略是必要的。小鼠（小家鼠）是目前生物医学研究中最常用的实验动物。对动物进行实验操作通常需要麻醉和/或镇痛，以实现充分的固定，并减轻应激或疼痛。小鼠麻醉具有挑战性，原因有几个，包括动物的体型、代谢率以及体温过低和低血糖的高风险。此外，麻醉剂会影响生理参数，进一步干扰实验结果。小动物成像程序在生物医学研究中越来越多地被使用，这既是因为这些动物允许进行体内监测，也是因为它们很容易用于纵向和非侵入性研究，以及对疾病演变和新疗法效果的研究。麻醉必须适应成像技术、操作时长和研究目的。本文的目的是综述现有的关于小鼠分子成像研究中采用的麻醉方案的文献，并报告我们的经验。
