Korendovych Ivan V
Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY, 13244, USA.
Methods Mol Biol. 2018;1685:15-23. doi: 10.1007/978-1-4939-7366-8_2.
This mini review gives an overview over different design approaches and methodologies applied in rational and semirational enzyme engineering. The underlying principles for engineering novel activities, enantioselectivity, substrate specificity, stability, and pH optimum are summarized.
本综述概述了合理和半合理酶工程中应用的不同设计方法和策略。总结了工程化新活性、对映选择性、底物特异性、稳定性和最适pH值的基本原理。
