College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No.6 Tongwei Road Nanjing, P. R. China, 210095.
Department of Rural Development, Nanjing Agricultural University, No.6 Tongwei Road Nanjing, P. R. China, 210095.
Bioinformatics. 2018 Feb 15;34(4):708-709. doi: 10.1093/bioinformatics/btx633.
High throughput bisulfite sequencing (BS-seq) is an important technology to generate single-base DNA methylomes in both plants and animals. In order to accelerate the data analysis of BS-seq data, toolkits for visualization are required.
ViewBS, an open-source toolkit, can extract and visualize the DNA methylome data easily and with flexibility. By using Tabix, ViewBS can visualize BS-seq for large datasets quickly. ViewBS can generate publication-quality figures, such as meta-plots, heat maps and violin-boxplots, which can help users to answer biological questions. We illustrate its application using BS-seq data from Arabidopsis thaliana.
ViewBS is freely available at: https://github.com/xie186/ViewBS.
Supplementary data are available at Bioinformatics online.
高通量亚硫酸氢盐测序（BS-seq）是在植物和动物中生成单碱基 DNA 甲基化组的重要技术。为了加速 BS-seq 数据的数据分析，需要可视化工具包。
ViewBS 是一个开源工具包，可以轻松、灵活地提取和可视化 DNA 甲基化组数据。通过使用 Tabix，ViewBS 可以快速可视化大型数据集的 BS-seq。ViewBS 可以生成出版质量的图形，如图谱、热图和小提琴箱线图，这可以帮助用户回答生物学问题。我们使用来自拟南芥的 BS-seq 数据来说明其应用。
ViewBS 可在以下网址免费获取：https://github.com/xie186/ViewBS。
补充数据可在《生物信息学》在线获取。
