Du Kangxi, Wu Jiabing, Wang Jiachen, Xie Wenhao, Yin Liufan, Li Xiang, Li Chao, Dong Aiwu
State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, PR China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, PR China.
Nat Commun. 2024 Dec 30;15(1):10919. doi: 10.1038/s41467-024-55387-4.
The INO80 chromatin remodeling complex plays a critical role in shaping the dynamic chromatin environment. The diverse functions of the evolutionarily conserved INO80 complex have been widely reported. However, the role of INO80 in modulating the histone variant H2A.Z is controversial. Moreover, whether INO80 helps regulate heterochromatin remains unknown. Here, we characterize the regulatory effects of OsINO80 on protein-coding genes and transposable elements (TEs) in rice. Upon OsINO80 overexpression in rice, we found three types of OsINO80-occupied regions with different chromatin signatures: type I (enriched with H2A.Z), type II (enriched with H3K9me2), and type III (deficient in H2A.Z/H3K9me2). Loss of OsINO80 results in a decrease in H3K27me3, but not H2A.Z, at type I regions as well as a decrease in H3K9me2 at type II regions, which correlates with TE activation and transposition. Our findings reveal that OsINO80 facilitates H3K27me3 establishment, promotes H3K9me2 deposition, and maintains TE silencing.
INO80染色质重塑复合体在塑造动态染色质环境中起着关键作用。进化上保守的INO80复合体的多种功能已被广泛报道。然而，INO80在调节组蛋白变体H2A.Z方面的作用存在争议。此外，INO80是否有助于调节异染色质仍不清楚。在这里，我们描述了水稻中OsINO80对蛋白质编码基因和转座元件（TEs）的调控作用。在水稻中过表达OsINO80后，我们发现了三种具有不同染色质特征的OsINO80占据区域：I型（富含H2A.Z）、II型（富含H3K9me2）和III型（缺乏H2A.Z/H3K9me2）。OsINO80的缺失导致I型区域的H3K27me3减少，但H2A.Z没有减少，以及II型区域的H3K9me2减少，这与TE的激活和转座相关。我们的研究结果表明，OsINO80促进H3K27me3的建立，促进H3K9me2的沉积，并维持TE的沉默。
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