Kort W J, Bijma A M, Hekking I M
Laboratory for Experimental Surgery, Erasmus University, Rotterdam, The Netherlands.
Clin Exp Metastasis. 1989 Jan-Feb;7(1):117-25. doi: 10.1007/BF02057186.
At -1, 0, +1 weeks from tumor inoculation, carrageenan-impregnated cotton sponges were subcutaneously implanted. Tumor BN472, a malignant adenocarcinoma, was transplanted in syngeneic Brown Norway female rats, either subcutaneously or intravenously. Plasma eicosanoid values (prostaglandin-E2, thromboxane-B2 and 6-keto-prostaglandin-F1 alpha) were determined as well as the cellular immune response (natural killer activity, concanavalin-A and phytohemagglutinin stimulation of spleen lymphocytes). Primary tumor growth and the number of tumor foci in the lungs were measured as parameters of tumor growth and dissemination. No statistically significant differences were observed in primary tumor growth. However, the number of metastatic foci in the lungs of rats in which the tumor was implanted subcutaneously, as well as those in which the tumor was inoculated intravenously, was significantly reduced in those that had carrageenan implanted one week after tumor inoculation. In all other carrageenan-treated groups only slightly reduced numbers of metastatic foci were seen. In those rats with a decreased number of tumor metastatic foci in the lungs, no correlation could be shown with either altered plasma prostaglandin levels, or cellular immune response.
在接种肿瘤后的-1、0、+1周，将浸有角叉菜胶的棉海绵皮下植入。将恶性腺癌肿瘤BN472移植到同基因的雌性棕色挪威大鼠体内，移植方式为皮下或静脉注射。测定血浆类花生酸值（前列腺素-E2、血栓素-B2和6-酮-前列腺素-F1α）以及细胞免疫反应（自然杀伤活性、伴刀豆球蛋白A和植物血凝素对脾淋巴细胞的刺激）。测量原发性肿瘤生长和肺内肿瘤病灶数量，作为肿瘤生长和扩散的参数。在原发性肿瘤生长方面未观察到统计学上的显著差异。然而，在肿瘤接种一周后植入角叉菜胶的大鼠中，皮下植入肿瘤以及静脉接种肿瘤的大鼠肺内转移灶数量显著减少。在所有其他角叉菜胶处理组中，仅观察到转移灶数量略有减少。在肺内肿瘤转移灶数量减少的大鼠中，未发现与血浆前列腺素水平改变或细胞免疫反应有相关性。
