Abulizi Palida, Zhou Xiao-Hui, Keyimu Kabinuer, Luo Mei, Jin Feng-Qing
No1 Cadre Wards Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China.
Oncotarget. 2017 Sep 6;8(43):75174-75185. doi: 10.18632/oncotarget.20655. eCollection 2017 Sep 26.
The secretive Klotho protein is an anti-aging regulatory hormone that plays a physiological role in many target organs. The present study aims to investigate the correlation between gene and mild cognitive impairment (MCI) in Uygur and Han populations in Xinjiang. From July 2008 to April 2014, stratified random multistage cluster sampling was used in combination with the methods of on-site questionnaire and household survey to conduct a cross-sectional MCI investigation on selected Uygur and Han subjects aged over 60 years old in Xinjiang region. 323 Uygur and Han MCI patients were randomly selected and matched with 343 subjects in the normal control group. SNaPshot technique was used to detect the polymorphisms of gene. A case-control associated analysis was conducted to analyze the genotype and allele frequencies of single nucleotide polymorphisms (SNPs) in the MCI group and the normal control group. The polymorphisms of rs1207568 and rs9536314/rs9527025 loci in gene were different among MCI populations in Xinjiang, and after group assignments based on ethnic background, the polymorphisms of rs1207568 and rs9536314/rs9527025 loci were associated with the Uygur MCI population and were not relevant to the Han MIC population. The frequencies of mutational alleles of rs9536314/rs9527025 locus in the Uygur population were significantly higher than those in the Han population. The genotype and allele frequencies of rs1207568 locus in the Uygur and Han populations were similar. The polymorphisms of rs1207568 and rs9536314/rs9527025 loci in gene may be associated with the Uygur MCI population in Xinjiang.
神秘的klotho蛋白是一种抗衰老调节激素，在许多靶器官中发挥生理作用。本研究旨在探讨新疆维吾尔族和汉族人群中该基因与轻度认知障碍（MCI）之间的相关性。2008年7月至2014年4月，采用分层随机多阶段整群抽样方法，结合现场问卷调查和家庭调查方法，对新疆地区60岁以上的维吾尔族和汉族受试者进行横断面MCI调查。随机选取323例维吾尔族和汉族MCI患者，并与343例正常对照组受试者进行匹配。采用SNaPshot技术检测该基因的多态性。进行病例对照关联分析，分析MCI组和正常对照组单核苷酸多态性（SNP）的基因型和等位基因频率。新疆MCI人群中该基因rs1207568和rs9536314/rs9527025位点的多态性存在差异，按民族背景分组后，rs1207568和rs9536314/rs9527025位点的多态性与维吾尔族MCI人群相关，与汉族MCI人群无关。维吾尔族人群中rs9536314/rs9527025位点的突变等位基因频率显著高于汉族人群。维吾尔族和汉族人群中rs1207568位点的基因型和等位基因频率相似。该基因rs1207568和rs9536314/rs9527025位点的多态性可能与新疆维吾尔族MCI人群有关。
