Key Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular-imaging, Department of Bioinformatics and Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, China.
Sci Rep. 2017 Oct 31;7(1):14393. doi: 10.1038/s41598-017-14807-w.
The single-cell analysis is becoming a powerful method for early detection of the abnormal variant in tissues, especially for profiling a small number of heterogeneous cells. With the advancement of sequencing technologies, many types of non-coding elements including miRNAs and lncRNAs which shed light on their heterogeneous patterns and functions among cells, have been profiled at the single-cell level. However, the complete picture of circRNA profile at single-cell level is still lacking. In this study, RNA-Seq data obtained from single HEK293T cells have been used to analyze expressions and functions of heterogeneous circRNA profiles. The enrichment patterns of circRNAs, interactions with miRNAs and pathways such as ErbB signaling pathway and protein processing in endoplasmic reticulum, have also been investigated. The results showed that circRNAs had a specific distribution pattern which was implicated with expression, miRNA and functional profiles at single-cell level. This assessment study of the expressions and functions of circRNAs at single-cell level shed light on heterogeneities among single cells.
单细胞分析正成为一种强大的方法，用于早期检测组织中的异常变异，特别是用于分析少数异质细胞。随着测序技术的进步，许多类型的非编码元件，包括 miRNA 和 lncRNA，已经在单细胞水平上对其异质模式和功能进行了分析。然而，circRNA 在单细胞水平上的完整图谱仍然缺乏。在这项研究中，从单个 HEK293T 细胞中获得的 RNA-Seq 数据被用于分析异质 circRNA 图谱的表达和功能。还研究了 circRNAs 的富集模式、与 miRNA 的相互作用以及 ErbB 信号通路和内质网蛋白加工等途径。结果表明，circRNAs 具有特定的分布模式，与单细胞水平的表达、miRNA 和功能图谱有关。这项对 circRNAs 在单细胞水平上的表达和功能的评估研究，揭示了单细胞之间的异质性。
