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氯化锂可诱导人永生化肾Vm细胞分化为多巴胺能神经元。

Lithium Chloride can Induce Differentiation of Human Immortalized RenVm Cells into Dopaminergic Neurons.

作者信息

Soleimani Mitra, Ghasemi Nazem

机构信息

Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Avicenna J Med Biotechnol. 2017 Oct-Dec;9(4):176-180.

PMID:29090066
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5650734/
Abstract

BACKGROUND

Stem cell-based therapy is a novel strategy for the treatment of neurodegenerative diseases. The transplantation of fully differentiated cells instead of stem cells in order to decrease serious adverse complications of stem cell therapy is a new idea. In this study, the effect of lithium chloride on dopaminergic differentiation of human immortalized RenVm cells was investigated in order to access a population of fully differentiated cells for transplantation in Parkinson disease.

METHODS

The immortalized RenVm cells were induced to dopaminergic differentiation using a neurobasal medium supplemented with N2 and different concentrations (1, 3, 6 ) of Lithium Chloride (LiCl) for 4, 8 and 12 days. The efficiency of dopaminergic differentiation was evaluated using immunocytochemistry and western blot techniques for tyrosine hydroxylase and β-catenin marker expression.

RESULTS

Our results indicated that LiCl can promote dopaminergic differentiation of RenVm cells in a dose-dependent manner.

CONCLUSION

It can be concluded that LiCl is able to facilitate dopaminergic differentiation of cultured cells by affecting Wnt-frizzled signaling pathway.

摘要

背景

基于干细胞的疗法是治疗神经退行性疾病的一种新策略。移植完全分化的细胞而非干细胞以减少干细胞治疗的严重不良并发症是一个新想法。在本研究中，研究了氯化锂对人永生化RenVm细胞多巴胺能分化的影响，以便获得用于帕金森病移植的完全分化细胞群体。

方法

使用补充有N2和不同浓度（1、3、6）氯化锂（LiCl）的神经基础培养基将永生化RenVm细胞诱导多巴胺能分化4、8和12天。使用免疫细胞化学和蛋白质印迹技术评估酪氨酸羟化酶和β-连环蛋白标志物表达，以评价多巴胺能分化的效率。

结果

我们的结果表明，LiCl可剂量依赖性地促进RenVm细胞的多巴胺能分化。

结论

可以得出结论，LiCl能够通过影响Wnt-卷曲信号通路促进培养细胞的多巴胺能分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/564245f8b9aa/AJMB-9-176-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/7a263f07ab7c/AJMB-9-176-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/d0a3e918336f/AJMB-9-176-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/966966ad4f60/AJMB-9-176-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/6ef8f0685613/AJMB-9-176-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/564245f8b9aa/AJMB-9-176-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/7a263f07ab7c/AJMB-9-176-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/d0a3e918336f/AJMB-9-176-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/966966ad4f60/AJMB-9-176-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/6ef8f0685613/AJMB-9-176-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c0/5650734/564245f8b9aa/AJMB-9-176-g005.jpg

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