Wang Yan-Fang, Li Ge, Tang Ling, Yang Chun-Yong, Li Rong-Ying
Yunnan Branch, Institute of Medicinal Plant, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Jinghong 666100, China.
Zhongguo Zhong Yao Za Zhi. 2017 May;42(10):1860-1864. doi: 10.19540/j.cnki.cjcmm.2017.0077.
In order to study the effect elements of anthers opening-closing movement of Paris polyphylla var. yunnanensis, and its cell morphology regulatory mechanism. Anthers daily opening in the morning and closing in the evening and its corresponding ecological elements changes were recorded. Different light, temperature, humidity experiment and artificial rainfall experiment were designed to observe the effect on anthers opening-closing movement, paraffin sections were made to observe the cell morphology change when the anthers daily opening and closing. The result showed that the movement of anthers daily opening and closing was regulated by ecological elements. The overall trend was high temperature and strong light, low humidity was favorable for anther opening, and low temperature, weak light, high humidity was favorable for anther closing. In this experiment, the effect of these ecological elements on the movement of anthers opening from strong to weak was humidity, temperature, light. The effect of these ecological elements on the movement of anthers closing from strong to weak was light, humidity, temperature. The direct contact of the raindrops causes the rapid closing of the anthers in the rain. Observing the cell morphology change when anthers opening and closing, it was a pollen sac dehydration and water-absorption process, different light, temperature and humidity conditions induce different physiological activities in the cell, which caused the cell osmotic pressure change, eventually resulting in anther opening and closing movement. But anthers closing caused by the rain was a simple physical adjustment process, the raindrops fell on the anthers directly, which caused anthers soaked water and change of the cell osmotic pressure, then resulting in anther closing.
为研究滇重楼花药开合运动的影响因素及其细胞形态调控机制，记录了滇重楼花药每日早开晚合及其相应生态因子的变化。设计了不同光照、温度、湿度实验及人工降雨实验，观察其对花药开合运动的影响，并制作石蜡切片观察花药每日开合时的细胞形态变化。结果表明，花药的每日开合运动受生态因子调控。总体趋势是高温、强光、低湿度有利于花药开放，低温、弱光、高湿度有利于花药闭合。本实验中，这些生态因子对花药开放运动影响从强到弱依次为湿度、温度、光照；对花药闭合运动影响从强到弱依次为光照、湿度、温度。雨滴直接接触导致花药在雨中迅速闭合。观察花药开合时的细胞形态变化，发现其为花粉囊脱水与吸水过程，不同的光照、温度和湿度条件诱导细胞内不同的生理活动，进而引起细胞渗透压变化，最终导致花药开合运动。但降雨导致的花药闭合是一个简单的物理调节过程，雨滴直接落在花药上，使其浸水，细胞渗透压改变，从而导致花药闭合。
