Su Jin-Song, Zhao Cai-Yun, Wen Jian, Liu Chuan, Xie Cai-Xiang, Zhang Yi
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical, Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2017 May;42(10):1865-1870. doi: 10.19540/j.cnki.cjcmm.2017.0078.
The study aims at providing a new suitable way to promote artificial cultivation, solving the problem of resources increasingly endangered wild medicine, and protecting the wild resources of Tibetan medicine. The content of quercetin,kaempferol and isorhamnetin was determined by HPLC. The correlation between flavonoids components and ecological factors was analyzed using partial least-squares regression (PLSR). Based on Maxent model combining using ArcGIS software, suitable regionalization for H.rhamnoides subsp. sinensis was studied.The results showed that the difference of quercetin,kaempferol and isorhamnetin content in samples from different regions were obvious. The main factors effecting quercetin content accumulation were the altitude andthe average monthly precipitation in January and August. The main factors effecting kaempferol accumulation were the altitude andthe average monthly precipitation in the coldest quarter and December. The main factors effecting isorhamnetin accumulation were the average monthly precipitation in August, January and the coldest quarter.The regional distribution suitability index for H.rhamnoides subsp. sinensis was 0-0.708. The suitable area 590 500 km², accounting for 6.13% of the total area. The preferably suitable area was 552 500 km², accounting for 5.73% of the total area.The methods used in the study is simple and feasible, the result is reliable which provide a new approach for Tibetan medicine resources sustainable exploitation and utilization.
本研究旨在提供一种新的适宜方法来促进人工栽培，解决野生药材资源日益濒危的问题，并保护藏药的野生资源。采用高效液相色谱法测定槲皮素、山柰酚和异鼠李素的含量。运用偏最小二乘回归（PLSR）分析黄酮类成分与生态因子之间的相关性。基于Maxent模型并结合ArcGIS软件，对中国沙棘亚种进行适宜性区划研究。结果表明，不同地区样品中槲皮素、山柰酚和异鼠李素含量差异明显。影响槲皮素含量积累的主要因素是海拔以及1月和8月的月平均降水量。影响山柰酚积累的主要因素是海拔以及最冷月和12月的月平均降水量。影响异鼠李素积累的主要因素是8月、1月和最冷月的月平均降水量。中国沙棘亚种的区域分布适宜性指数为0 - 0.708。适宜面积为590500平方千米，占总面积的6.13%。最优适宜面积为552500平方千米，占总面积的5.73%。本研究采用的方法简单可行，结果可靠，为藏药资源的可持续开发利用提供了新途径。
