Wang Kai, Sui Dan-Juan, Wang Chang-Suo, Yang Li, Ouyang Zhen, Chen Nai-Fu, Han Bang-Xing, Wei Yuan
School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China.
Zhongguo Zhong Yao Za Zhi. 2017 May;42(10):1945-1950. doi: 10.19540/j.cnki.cjcmm.2017.0082.
This study aims to investigate the protective effect of Dendrobium huoshanense, D.officinale(Huoshan), D.officinale(Yunnan), D.moniliforme and D. henanense on CCl4-induced hepatic damage in mice. C57BL/6 mice were randomly divided into control group, model group, high-dose(7.5 g•kg⁻¹) and low-dose (1.25 g•kg⁻¹) groups of the five Dendrobium. Each group was intragastrically administered with drugs for 2 weeks. The control group was intraperitoneally injected with Olive oil solution, while the other groups were intraperitoneally given 0.5%CCl4combined with Olive oil solution 2 h later after the last administration. Subsequently, ALT and AST activities in serum, SOD activities and MDA contents in liver tissues were determined in all groups 16 h later after administration. The liver index was calculated, and hepatic histopathological examination was performed. The mRNA expressions of IL-1β, IL-6 and TNF-α were analyzed by Real-time PCR. Compared with the CCl4 model group, the activities of ALT and AST in serum decreased significantly in the five different Dendrobium groups. Meanwhile, in liver tissues, the levels of MDA reduced obviously, while the SOD activities markedly increased. Furthermore, liver tissue damage induced by CCl4 was ameliorated according to the histopathological examination. IL-1β, IL-6 and TNF-α mRNA expressions in D.huoshanense-treated liver tissues were significantly decreased. In conclusion, the five different Dendrobium groups showed hepatoprotective effects on CCl4-induced acute liver injury in mice. However, there were differences among Dendrobium of different types and origins. The protect effect of D.huoshanense is the most obvious, and the order of the protective effect of the other Dendrobium from high to low is D.officinale(Yunnan), D. officinale(Huoshan), D.henanense and D.moniliforme. The differences between the different types of Dendrobium might be related to their chemical components.
本研究旨在探讨霍山石斛、铁皮石斛（霍山）、铁皮石斛（云南）、串珠石斛和河南石斛对四氯化碳诱导的小鼠肝损伤的保护作用。将C57BL/6小鼠随机分为对照组、模型组、五种石斛的高剂量（7.5 g•kg⁻¹）和低剂量（1.25 g•kg⁻¹）组。每组灌胃给药2周。对照组腹腔注射橄榄油溶液，而其他组在末次给药2小时后腹腔注射0.5%四氯化碳与橄榄油溶液。给药16小时后，测定所有组血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）活性、肝组织中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。计算肝脏指数，并进行肝脏组织病理学检查。通过实时荧光定量PCR分析白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的mRNA表达。与四氯化碳模型组相比，五种不同石斛组血清中ALT和AST活性显著降低。同时，肝组织中MDA水平明显降低，而SOD活性显著升高。此外，根据组织病理学检查，四氯化碳诱导的肝组织损伤得到改善。霍山石斛处理的肝组织中IL-1β、IL-6和TNF-α mRNA表达显著降低。综上所述，五种不同石斛组对四氯化碳诱导的小鼠急性肝损伤均有保肝作用。然而，不同类型和产地的石斛之间存在差异。霍山石斛的保护作用最明显，其他石斛保护作用由高到低的顺序为铁皮石斛（云南）、铁皮石斛（霍山）、河南石斛和串珠石斛。不同类型石斛之间的差异可能与其化学成分有关。
