Li Zhi-Qiang, Zhou Hong-Qiu, Ouyang Zhen, Dai Jun, Yue Qin, Wei Yuan, Han Bang-Xing
School of Pharmacy, Jiangsu University Zhenjiang 212013, China.
Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory Lu'an 237012, China College of Biotechnology and Pharmaceutical Engineering, West Anhui University Lu'an 237012, China.
Zhongguo Zhong Yao Za Zhi. 2021 Jan;46(2):298-305. doi: 10.19540/j.cnki.cjcmm.20201023.102.
The differences of the active ingredients in Dendrobium huoshanense of different growth years and their protective effects on acute liver injury were studied to provide evidence for optimizing harvest time. The contents of polysaccharides, total flavonoids and total alkaloids in D. huoshanense of different growth years were determined by UV spectrophotometry, and the contents of gigantol in D. huoshanense were determined by HPLC. C57 BL/6 mice were randomly divided into blank control group(saline), modeling group(saline), high-dose(7.5 g·kg(-1)) and low-dose(1.25 g·kg(-1)) groups of D. huoshanense of different growth years. Each group was intragastrically administered every day for 2 weeks. 500 mg·kg~(-1) paracetamol was injected intraperitoneally 2 h after last treatment except the control group. After 12 hours, the serum and liver tissues were collected to detect the activities of ALT and AST, and the levels of SOD and MDA. The hepatic histopathological examination was performed. The results showed that the chemical constituents of D. huo-shanense of different growth years were significantly different(P<0.05). The contents of polysaccharide and gigantol of D. huoshanense of 2 growth years were the highest. The contents of flavonoids and alkaloids of D. huoshanense of 3 growth years were the hig-hest, followed by the D. huoshanense of 2 growth years, and the lowest were that of 1 growth year. Compared with the modeling group, D. huoshanense of different growth years could decrease the activities of ALT and AST in serum. Meanwhile, the levels of MDA reduced significantly, while those of SOD increased markedly. Histopathological results suggested that all D. huoshanense samples were effective in the reduction of the necrosis of hepatocytes in different degrees. The results of the multi-component SPSS paired tests showed that polysaccharide and gigantol probably played a leading role in the liver protection effects, while D. huoshanense of 2 growth years showed the best efficacy. The optimal harvesting time of D. huoshanense is 2 growth years.
研究不同生长年限霍山石斛有效成分差异及其对急性肝损伤的保护作用，为优化采收时间提供依据。采用紫外分光光度法测定不同生长年限霍山石斛中多糖、总黄酮和总生物碱含量，高效液相色谱法测定霍山石斛中石斛酚含量。将C57 BL/6小鼠随机分为空白对照组（生理盐水）、模型组（生理盐水）、不同生长年限霍山石斛高剂量（7.5 g·kg⁻¹）和低剂量（1.25 g·kg⁻¹）组。每组每天灌胃给药，连续2周。除对照组外，末次给药后2 h腹腔注射500 mg·kg⁻¹对乙酰氨基酚。12 h后，采集血清和肝组织，检测ALT、AST活性及SOD、MDA水平，并进行肝脏组织病理学检查。结果表明，不同生长年限霍山石斛化学成分有显著差异（P<0.05）。2年生霍山石斛多糖和石斛酚含量最高。3年生霍山石斛黄酮和生物碱含量最高，其次是2年生霍山石斛，1年生霍山石斛最低。与模型组相比，不同生长年限霍山石斛均可降低血清中ALT和AST活性。同时，MDA水平显著降低，SOD水平显著升高。组织病理学结果表明，各霍山石斛样品均能不同程度减轻肝细胞坏死。多成分SPSS配对检验结果显示，多糖和石斛酚可能在保肝作用中起主导作用，2年生霍山石斛药效最佳。霍山石斛最佳采收时间为2年生。
