Liu Shu-Min, Zhang Ning, Yu Dong-Hua, Wang Yu, Zhou Qi, Lu Fang
Drug Safety Evaluation Center, Academy of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Zhongguo Zhong Yao Za Zhi. 2017 May;42(10):1971-1978. doi: 10.19540/j.cnki.cjcmm.20170224.011.
To explore the prevention and protection effect of Diosocorea nipponica (DNM)) on acute gouty arthritis (AGA) rats based on liver metabonomics, and find potential biomarkers and related pathways. AGA model rats were induced by monosodium urate crystal suspension. UPLC-TOF-MS coupled with pattern recognition technique was employed to find out the potential biomarkers and related metabolic pathways. Eleven common potential biomarkers were identified. Among the potential intervention targets in normal rats given by DNM, 4 biomarkers were up-regulated, and the other 4 targets were down regulated. Among the potential intervention targets in AGA rats given by DNM, 5 metabolites were up-regulated by MSU and 5 metabolites were down regulated. The abnormal expression levels of adenosine monophosphate, 5-methyltetrahydrofolic acid, oxidized glutathione, hypoxanthine, docosahexaenoic acid, glutathione, uridine diphosphate glucose and inosine could be corrected by DNM extract. Three pathways were founded with greatest correlation, including purine metabolism, starch and sucrose metabolism and glutathione metabolism. Therefore, it could be inferred that D. nipponica has the effect for anti-acute gouty arthritis by intervening endogenous metabolites from the liver under physiological condition and acute gouty arthritis condition.
基于肝脏代谢组学探讨穿山龙对急性痛风性关节炎（AGA）大鼠的预防和保护作用，并寻找潜在生物标志物及相关通路。采用尿酸钠晶体悬液诱导建立AGA模型大鼠。运用超高效液相色谱-飞行时间质谱联用（UPLC-TOF-MS）结合模式识别技术找出潜在生物标志物及相关代谢通路。共鉴定出11种常见潜在生物标志物。在给予穿山龙的正常大鼠潜在干预靶点中，4种生物标志物上调，另外4种靶点下调。在给予穿山龙的AGA大鼠潜在干预靶点中，5种代谢物被尿酸钠上调，5种代谢物下调。穿山龙提取物可纠正单磷酸腺苷、5-甲基四氢叶酸、氧化型谷胱甘肽、次黄嘌呤、二十二碳六烯酸、谷胱甘肽、尿苷二磷酸葡萄糖和肌苷的异常表达水平。发现了3条相关性最大的通路，包括嘌呤代谢、淀粉和蔗糖代谢以及谷胱甘肽代谢。因此，可以推断穿山龙在生理状态和急性痛风性关节炎状态下通过干预肝脏内源性代谢物而具有抗急性痛风性关节炎的作用。
