Sobral Laureana V, Melo Kelly N, Souza Cleciana M, Silva Sílvio F, Silva Gilvania L R, Silva Andressa L F, Wanderley Katharine A A, Oliveira Idjane S, Cruz Roberta
Laboratório de Microbiologia e Imunologia, Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Rua Alto do Reservatório, s/n, Bela Vista, 55608-680 Vitória de Santo Antão, PE, Brazil.
Departamento de Micologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brazil.
An Acad Bras Cienc. 2017;89(3 Suppl):2327-2340. doi: 10.1590/0001-3765201720160903. Epub 2017 Oct 26.
To the fungal microbiota the UFPE and biotechnological potential enzymatic and antimicrobial production. Air conditioned environments were sampled using a passive sedimentation technique, the air I ratio and the presence of aflatoxigenic strains evaluated for ANVISA. Icelles were to determine the enzymatic activity of lipase, amylase and protease metabolic liquids to determine antimicrobial activity. Diversity was observed in all CAV environments, CFU/m3 ranged from 14 to 290 and I/E ratio from 0.1 to 1.5. The of the fungal genera were: Aspergillus (50%), Penicillium (21%), Talaromyces (14%), Curvularia and Paecilomyces (7% each). Aspergillus sydowii (Bainier & Sartory) Thom & Church presented enzymatic activity and the Talaromyces purpureogenus Samson, Yilmaz, Houbraken, Spierenb., Seifert, Peterson, Varga & Frisvad presented antibacterial activity against all bacteria that all environments present fungal species biodiversity no toxigenic or pathogenic fungi were found, according to ANVISA legislation for conditioned environments and airborne filamentous fungi present potential for enzymatic and antimicrobial activity.
关于真菌微生物群,UFPE以及酶和抗菌产品的生物技术潜力。使用被动沉降技术对空调环境进行采样,评估空气中的I比率以及黄曲霉毒素产生菌株的存在情况以供巴西卫生监督局(ANVISA)参考。测定细胞中脂肪酶、淀粉酶和蛋白酶代谢液的酶活性,以确定抗菌活性。在所有空调环境中均观察到多样性,每立方米菌落形成单位(CFU/m3)范围为14至290,I/E比率为0.1至1.5。真菌属包括:曲霉属(50%)、青霉属(21%)、篮状菌属(14%)、弯孢属和拟青霉属(各占7%)。烟曲霉(Aspergillus sydowii (Bainier & Sartory) Thom & Church)呈现酶活性,而紫篮状菌(Talaromyces purpureogenus Samson, Yilmaz, Houbraken, Spierenb., Seifert, Peterson, Varga & Frisvad)对所有细菌均呈现抗菌活性。所有环境中均存在真菌物种生物多样性,根据巴西卫生监督局针对空调环境的法规,未发现产毒或致病真菌,且空气传播丝状真菌具有酶活性和抗菌活性的潜力。