在 3.9Å 冷冻电镜图中构建一种未知折叠的北方湖泊病毒的原子模型。
Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map.
机构信息
Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK.
Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK; Diamond Light Source Ltd, Harwell Science & Innovation Campus, Didcot OX11 0DE, UK.
出版信息
J Struct Biol. 2018 Apr;202(1):94-99. doi: 10.1016/j.jsb.2017.10.010. Epub 2017 Oct 27.
We report here the protocol adopted to build the atomic model of the newly discovered virus FLiP (Flavobacterium infecting, lipid-containing phage) into 3.9 Å cryo-electron microscopy (cryo-EM) maps. In particular, this report discusses the combination of density modification procedures, automatic model building and bioinformatics tools applied to guide the tracing of the major capsid protein (MCP) of this virus. The protocol outlined here may serve as a reference for future structural determination by cryo-EM of viruses lacking detectable structural homologues.
我们在此报告了构建新发现的病毒 FLiP（感染黄杆菌的含脂噬菌体）原子模型的方案，该模型基于 3.9Å 冷冻电镜（cryo-EM）图谱。特别是，本报告讨论了密度修正程序、自动模型构建和生物信息学工具的结合，这些工具用于指导该病毒主要衣壳蛋白（MCP）的追踪。这里概述的方案可能为未来缺乏可检测结构同源物的病毒的 cryo-EM 结构测定提供参考。
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