Lukášová Radka, Kobédová Kateřina, Halajian Ali, Bártová Eva, Murat Jean-Benjamin, Rampedi Kgethedi Michael, Luus-Powell Wilmien J
University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Hygiene and Ecology, Department of Biology and Wildlife Diseases, Palackého tř. 1946/1, 612 42 Brno, Czech Republic.
Department of Biodiversity, University of Limpopo, Sovenga 0727, South Africa.
Acta Trop. 2018 Feb;178:93-96. doi: 10.1016/j.actatropica.2017.10.029. Epub 2017 Oct 29.
There are not any records on the detection of Toxoplasma gondii and Neospora caninum in tissues of wild birds in the African continent. The aim of the study was to investigate the occurrence of DNA from these protozoan parasites in brain tissue samples collected in years 2014-2015 from 110 wild and domestic birds of 15 orders. Birds came mainly from the province of Limpopo (n=103); the other seven birds came from other five provinces of South Africa. Parasite DNAs were detected by PCR in animal brains. While all samples were negative for N. caninum, T. gondii DNA was detected in three (2.7%) birds: a Red-eyed Dove (Streptopelia semitorquata), a Laughing Dove (S. senegalensis) and a Southern-Yellow-billed Hornbill (Tockus leucomelas), all from Limpopo province. Positive samples were selected for genotyping by a 15 microsatellite markers method in a single multiplex PCR assay. Only the sample from the Red-eyed Dove was successfully genotyped and characterized as type II. This is the first detection of T. gondii in tissue of native African wild birds and the first study focusing on N. caninum in birds from South Africa.
在非洲大陆，尚无关于野生鸟类组织中弓形虫和犬新孢子虫检测的记录。本研究的目的是调查2014 - 2015年从15个目110只野生和家养鸟类收集的脑组织样本中这些原生动物寄生虫DNA的存在情况。鸟类主要来自林波波省（n = 103）；另外7只鸟来自南非其他五个省。通过PCR在动物脑组织中检测寄生虫DNA。虽然所有样本犬新孢子虫均为阴性，但在3只（2.7%）鸟类中检测到了弓形虫DNA：一只红-eyed Dove（Streptopelia semitorquata）、一只斑颊哀鸽（S. senegalensis）和一只南非黄嘴犀鸟（Tockus leucomelas），均来自林波波省。通过单一多重PCR检测法，使用15个微卫星标记方法对阳性样本进行基因分型。只有来自红-eyed Dove的样本成功进行了基因分型，并被鉴定为II型。这是首次在非洲本土野生鸟类组织中检测到弓形虫，也是首次对南非鸟类中的犬新孢子虫进行研究。
