Vector Control Department, Institute of Tropical Medicine "Pedro Kourí," Havana, Cuba.
Faculty of Health Sciences, Havana, Cuba.
Am J Trop Med Hyg. 2021 Nov 8;106(2):582-584. doi: 10.4269/ajtmh.21-0710.
Transovarial transmission (TOT) of dengue virus (DENV) in Aedes spp. is an important mechanism for DENV maintenance in nature and may be important in initiating outbreaks. The objective of this study was to explore the occurrence of TOT in wild Aedes albopictus populations in Cuba. Mosquito larvae were collected in Cotorro municipality, Havana, Cuba, and identified to species. Fifteen pools of Ae. albopictus each containing 30 larvae were processed for DENV detection by using conventional reverse transcription polymerase chain reaction (RT-PCR) and nested PCR. Four out of 15 pools processed were positive for DENV-3, but no other DENV serotype was detected. This is the first time TOT of DENV detected in Cuban field populations of Ae. albopictus, and this suggests that this species may be an important vector of DENV in Cuba.
经卵传播（TOT）是登革病毒（DENV）在埃及伊蚊属中自然维持的重要机制，可能对引发疫情很重要。本研究的目的是探索古巴野生白纹伊蚊种群中 TOT 的发生情况。在古巴哈瓦那的科托罗市收集蚊子幼虫，并进行物种鉴定。用常规逆转录聚合酶链反应（RT-PCR）和巢式 PCR 对 15 个每个含有 30 只幼虫的白纹伊蚊幼虫进行 DENV 检测。在处理的 15 个幼虫中，有 4 个呈 DENV-3 阳性，但未检测到其他 DENV 血清型。这是首次在古巴野外白纹伊蚊种群中检测到 DENV 的 TOT，这表明该物种可能是古巴 DENV 的重要传播媒介。
