Laboratory of Microsystems LMIS4, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Nat Commun. 2017 Nov 1;8(1):1239. doi: 10.1038/s41467-017-01419-1.
Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. Current approaches using microdialysis catheters are however drastically limited in term of temporal resolution. Here we show a functional in vivo validation of a droplet collection system included at the tip of a neural probe. The system comprises an advanced droplet formation mechanism which enables the collection of neurochemicals present in the brain ECF at high-temporal resolution. The probe was implanted in a rat brain and could successfully collect fluid samples organized in a train of droplets. A microfabricated target plate compatible with most of the surface-based detection methods was specifically developed for sample analysis. The time-resolved brain-fluid samples are analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The results provide a time evolution picture of the cerebral tissues neurochemical composition for selected elements known for their involvement in neurodegenerative diseases.
直接采集细胞外液 (ECF) 在监测神经疾病方面起着核心作用。然而，目前使用微透析导管的方法在时间分辨率方面受到极大限制。在这里，我们展示了一种位于神经探针尖端的液滴收集系统的功能体内验证。该系统包括一种先进的液滴形成机制，可实现以高时间分辨率收集存在于脑 ECF 中的神经化学物质。该探针被植入大鼠大脑中，并成功收集了以液滴形式存在的流体样本。专门开发了一种与大多数基于表面的检测方法兼容的微加工目标板，用于样本分析。使用激光烧蚀电感耦合等离子体质谱法 (LA-ICP-MS) 对时分辨脑液样本进行分析。结果为选定元素的脑组织神经化学组成的时间演变图提供了信息，这些元素已知与神经退行性疾病有关。
