Laboratory of Bioenergetics and Biomembranes, Department of Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Science, 3 Pasteur St, 02-993, Warsaw, Poland.
Sci Rep. 2017 Nov 1;7(1):14864. doi: 10.1038/s41598-017-14964-y.
Mitochondrial dysfunctions lead to the generation of signalling mediators that influence the fate of that organelle. Mitochondrial dynamics and their positioning within the cell are important elements of mitochondria-nucleus communication. The aim of this project was to examine whether mitochondrial shape, distribution and fusion/fission proteins are involved in the mitochondrial stress response in a cellular model subjected to specifically designed chronic mitochondrial stress: WT human osteosarcoma cells as controls, NARP cybrid cells as mild chronic stress and Rho0 as severe chronic stress. We characterized mitochondrial distribution in these cells using confocal microscopy and evaluated the level of proteins directly involved in the mitochondrial dynamics and their regulation. We found that the organization of mitochondria within the cell is correlated with changes in the levels of proteins involved in mitochondrial dynamics and proteins responsible for regulation of this process. Induction of the autophagy/mitophagy process, which is crucial for cellular homeostasis under stress conditions was also shown. It seems that mitochondrial shape and organization within the cell are implicated in retrograde signalling in chronic mitochondrial stress.
线粒体功能障碍会产生信号介质，从而影响该细胞器的命运。线粒体动力学及其在细胞内的定位是线粒体-核通讯的重要因素。本项目的目的是研究在细胞模型中，线粒体的形状、分布和融合/分裂蛋白是否参与特定设计的慢性线粒体应激的线粒体应激反应：WT 人骨肉瘤细胞作为对照，NARP 细胞作为轻度慢性应激，Rho0 作为重度慢性应激。我们使用共聚焦显微镜来描述这些细胞中的线粒体分布，并评估直接参与线粒体动力学及其调节的蛋白质的水平。我们发现，细胞内线粒体的组织与参与线粒体动力学的蛋白质和负责调节该过程的蛋白质的水平变化相关。还显示了应激条件下细胞稳态所必需的自噬/线粒体自噬过程的诱导。似乎细胞内线粒体的形状和组织与慢性线粒体应激中的逆行信号有关。
