Na Buqi, Zhang Hong, Wang Guangfa, Dai Li, Xia Guoguang
Department of Respiratory and Critical Care Medicine, Beijing Jishuitan Hospital, Beijing 100035, China.
Department of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing 100034, China.
Can Respir J. 2017;2017:8530352. doi: 10.1155/2017/8530352. Epub 2017 Sep 28.
TWIK-related acid-sensitive potassium channel 1 (TASK-1) is closely related to respiratory central control and neuronal injury. We investigated the effect of MV on TASK-1's functions and explored the mechanism using a rat model.
Male Sprague-Dawley rats were randomized to three groups: (1) high tidal volume (HVt): MV for four hours with Vt at 10 mL/kg; (2) low Vt (LVt): MV for four hours with Vt at 5 mL/kg; (3) basal (BAS): anesthetized and unventilated animals. We measured lung histology and plasma and brain levels of proteins (IL-6, TNF-, and S-100B) and determined TASK-1 levels in rat brainstems as a marker of respiratory centre activity.
The LISs (lung injury scores) were significantly higher in the HVt group. Brain inflammatory cytokines levels were different to those in serum. TASK-1 levels were significantly lower in the MV groups ( = 0.002) and the HVt group tended to have a lower level of TASK-1 than the LVt group.
MV causes not only lung injury, but also brain injury. MV affects the regulation of the respiratory centre, perhaps causing damage to it. Inflammation is probably not the main mechanism of ventilator-related brain injury.
TWIK相关酸敏感钾通道1（TASK-1）与呼吸中枢控制和神经元损伤密切相关。我们使用大鼠模型研究了机械通气（MV）对TASK-1功能的影响并探讨了其机制。
将雄性Sprague-Dawley大鼠随机分为三组：（1）高潮气量（HVt）组：以10 mL/kg的潮气量进行4小时机械通气；（2）低Vt（LVt）组：以5 mL/kg的潮气量进行4小时机械通气；（3）基础（BAS）组：麻醉且未通气的动物。我们测量了肺组织学以及血浆和脑内蛋白质（IL-6、TNF-α和S-100B）水平，并测定大鼠脑干中TASK-1水平作为呼吸中枢活动的标志物。
HVt组的肺损伤评分（LISs）显著更高。脑内炎性细胞因子水平与血清中的不同。MV组的TASK-1水平显著更低（P = 0.002），且HVt组的TASK-1水平倾向于低于LVt组。
机械通气不仅会导致肺损伤，还会导致脑损伤。机械通气影响呼吸中枢的调节，可能对其造成损害。炎症可能不是呼吸机相关性脑损伤的主要机制。
