Kim Sehwan, Jung Un Ju, Oh Yong-Seok, Jeon Min-Tae, Kim Hyung-Jun, Shin Won-Ho, Hong Jungwan, Kim Sang Ryong
School of Life Sciences, BK21 plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Korea.
Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Korea.
Exp Neurobiol. 2017 Oct;26(5):266-277. doi: 10.5607/en.2017.26.5.266. Epub 2017 Oct 18.
Silibinin, an active constituent of silymarin extracted from milk thistle, has been previously reported to confer protection to the adult brain against neurodegeneration. However, its effects against epileptic seizures have not been examined yet. In order to investigate the effects of silibinin against epileptic seizures, we used a relevant mouse model in which seizures are manifested as status epilepticus, induced by kainic acid (KA) treatment. Silibinin was injected intraperitoneally, starting 1 day before an intrahippocampal KA injection and continued daily until analysis of each experiment. Our results indicated that silibinin-treatment could reduce seizure susceptibility and frequency of spontaneous recurrent seizures (SRS) induced by KA administration, and attenuate granule cell dispersion (GCD), a morphological alteration characteristic of the dentate gyrus (DG) in temporal lobe epilepsy (TLE). Moreover, its treatment significantly reduced the aberrant levels of apoptotic, autophagic and pro-inflammatory molecules induced by KA administration, resulting in neuroprotection in the hippocampus. Thus, these results suggest that silibinin may be a beneficial natural compound for preventing epileptic events.
水飞蓟宾是从水飞蓟中提取的水飞蓟素的一种活性成分，此前有报道称其能保护成人大脑免受神经退行性变的影响。然而，其对癫痫发作的影响尚未得到研究。为了研究水飞蓟宾对癫痫发作的影响，我们使用了一种相关的小鼠模型，在该模型中，癫痫发作表现为惊厥持续状态，由 kainic 酸（KA）处理诱导。水飞蓟宾在海马内注射 KA 前 1 天开始腹腔注射，并持续每日注射直至每个实验分析。我们的结果表明，水飞蓟宾治疗可降低 KA 给药诱导的癫痫易感性和自发性反复癫痫发作（SRS）的频率，并减轻颗粒细胞弥散（GCD），这是颞叶癫痫（TLE）中齿状回（DG）的一种形态学改变特征。此外，其治疗显著降低了 KA 给药诱导的凋亡、自噬和促炎分子的异常水平，从而在海马中产生神经保护作用。因此，这些结果表明水飞蓟宾可能是预防癫痫发作的一种有益天然化合物。
