Chandra Soumen, Chowdhuri Angshuman Ray, Laha Dipranjan, Sahu Sumanta Kumar
Department of Applied Chemistry, Indian Institute of Technology (ISM), Dhanbad, Jharkhand, India.
Department of Life Science and Biotechnology, Jadavpur University, Kolkata, India.
Luminescence. 2018 Mar;33(2):336-344. doi: 10.1002/bio.3418. Epub 2017 Nov 2.
A facile and novel strategy to synthesize nitrogen- and phosphorous-doped carbon dots (NPCDs) by single step pyrolysis method is described here. Citric acid is used as carbon source and di-ammonium hydrogen phosphate is used as both nitrogen and phosphorous sources, respectively. Through the extensive study on optical properties, morphology and chemical structures of the synthesized NPCDs, it is found that as-synthesized NPCDs exhibited good excitation-dependent luminescence property, spherical morphology and high stability. The obtained NPCDs are stable in aqueous medium and possess a quantum yield of 10.58%. In this work, a new assay method is developed to detect iodide ions using the synthesized NPCDs. Here, the inner filter effect is applied to detect the iodide ion and exhibited a wide linear response concentration range (10-60 μM) with a limit of detection (LOD) of 0.32 μM. Furthermore, the synthesized NPCDs are used for the selective detection of iron(III) (Fe ) ions and cell imaging. Fe ions sensing assay shows a detection range from 0.2 to 30 μM with a LOD of 72 nM. As an efficient photoluminescence sensor, the developed NPCDs have an excellent biocompatibility and low cytotoxicity, allowing Fe ion detection in HeLa cells.
本文描述了一种通过一步热解方法合成氮磷共掺杂碳点（NPCDs）的简便新颖策略。柠檬酸用作碳源，磷酸氢二铵分别用作氮源和磷源。通过对合成的NPCDs的光学性质、形态和化学结构进行广泛研究，发现合成的NPCDs表现出良好的激发依赖发光特性、球形形态和高稳定性。所获得的NPCDs在水性介质中稳定，量子产率为10.58%。在这项工作中，开发了一种使用合成的NPCDs检测碘离子的新检测方法。在此，利用内滤效应检测碘离子，其线性响应浓度范围宽（10 - 60 μM），检测限（LOD）为0.32 μM。此外，合成的NPCDs用于选择性检测铁（III）（Fe³⁺）离子和细胞成像。Fe³⁺离子传感检测显示检测范围为0.2至30 μM，LOD为72 nM。作为一种高效的光致发光传感器，所开发的NPCDs具有优异的生物相容性和低细胞毒性，能够在HeLa细胞中检测Fe³⁺离子。
