Micro-Nano System Research Centre & Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
Theranostic Macromolecules Research Center, School of Chemical Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Mikrochim Acta. 2019 Oct 31;186(11):736. doi: 10.1007/s00604-019-3872-0.
Pyrophosphate-modified carbon quantum dots (PP-CDs) are demonstrated to be a viable fluorescent nanoprobe for mercury(II) (Hg) detection. Hg reacts with the pyrophosphate groups on the surface of PP-CDs to form a non-fluorescent complex. This results in quenching of the green fluorescence which has excitation/emission peaks at 400/513 nm. Static quenching is shown to be the dominant mechanism. The probe works in 0.1 μM to 1.4 μM Hg concentration range, and the limit of detection is 2 nM. The PP-CDs were also used to visualize Hg inside human hepatocyte LO2 cells. Graphical abstract Schematic representation of pyrophosphate-modified carbon quantum dots (CDs) for selective and sensitive fluorometric determination of mercury(II). Hg(II) quenches the blue fluorescence of the CDs, and glutathione restores it. The method was used to detect Hg(II) in spiked tap water and inside cells.
焦磷酸修饰的碳量子点（PP-CDs）被证明是一种可行的用于汞（II）（Hg）检测的荧光纳米探针。Hg 与 PP-CDs 表面的焦磷酸基团反应，形成非荧光络合物。这导致在 400/513nm 的激发/发射峰处的绿色荧光猝灭。结果表明，静态猝灭是主要机制。该探针在 0.1μM 至 1.4μM Hg 浓度范围内工作，检测限为 2nM。还将 PP-CDs 用于可视化人肝细胞 LO2 细胞内的 Hg。 图表摘要 用于选择性和灵敏荧光测定汞（II）的焦磷酸修饰碳量子点（CDs）的示意性表示。Hg（II）猝灭 CD 的蓝色荧光，而谷胱甘肽将其恢复。该方法用于检测自来水中和细胞内的 Hg（II）。
