Dermatology, Venereology and Andrology Department, Ain Shams University, Cairo, Egypt.
Dermatology Department, Cairo University, Cairo, Egypt.
Pigment Cell Melanoma Res. 2018 Mar;31(2):330-336. doi: 10.1111/pcmr.12667. Epub 2017 Dec 1.
This cross-sectional multicenter study aimed to evaluate serum CXCL-10, as an activity marker for vitiligo, and compare it with other putative serum and tissue markers. Serum CXCL-10 was compared to interferon gamma (IFN-γ), interleukin 6 (IL-6), and IL-17 using ELISA in 55 non-segmental vitiligo patients (30 active and 25 stable) and 30 healthy controls. Marginal skin biopsy was taken for immunohistochemical evaluation of CD8+T cells and CXCL-10+ve cells. Serum levels of CXCL-10, IL-17, and IL-6 were elevated in all vitiligo patients compared to controls (p < .05). All investigated serum markers were higher in active versus stable vitiligo. Tissue expression of CXCL-10+ve cells and CD8+ve T cells was stronger in vitiligo patients compared to controls, and tissue CXCL-10+ve cell expression was stronger in active versus stable cases. Positive correlations were noted between the different serum and tissue markers. CXCL-10 was the most specific, whereas IL-6 was the most sensitive serum marker to distinguish active from stable disease.
本横断面多中心研究旨在评估血清 CXCL-10 作为白癜风的活性标志物，并将其与其他假定的血清和组织标志物进行比较。使用 ELISA 法比较了 55 例非节段性白癜风患者（30 例活动期和 25 例稳定期）和 30 名健康对照者血清中 CXCL-10 与干扰素γ（IFN-γ）、白细胞介素 6（IL-6）和白细胞介素 17（IL-17）的水平。对边缘皮肤进行免疫组织化学评估，以评估 CD8+T 细胞和 CXCL-10+ve 细胞。与对照组相比，所有白癜风患者的血清 CXCL-10、IL-17 和 IL-6 水平均升高（p<0.05）。与稳定期相比，活动期白癜风患者的所有检测血清标志物均升高。与对照组相比，白癜风患者皮肤组织中 CXCL-10+ve 细胞和 CD8+T 细胞的表达更强，且活动期病例皮肤组织中 CXCL-10+ve 细胞的表达强于稳定期。不同的血清和组织标志物之间存在正相关。CXCL-10 是最具特异性的，而 IL-6 是最敏感的血清标志物，可用于区分活动期和稳定期疾病。
