Alves Rosana, Mota Sandra, Silva Sónia, F Rodrigues Célia, P Brown Alistair J, Henriques Mariana, Casal Margarida, Paiva Sandra
a Centre of Molecular and Environmental Biology, Department of Biology , University of Minho , Braga , Portugal.
b Centre of Health and Environmental Research, School of Allied Health Sciences , Polytechnic Institute of Porto , Porto , Portugal.
Biofouling. 2017 Nov;33(10):943-954. doi: 10.1080/08927014.2017.1392514. Epub 2017 Nov 2.
Candida albicans has the ability to adapt to different host niches, often glucose-limited but rich in alternative carbon sources. In these glucose-poor microenvironments, this pathogen expresses JEN1 and JEN2 genes, encoding carboxylate transporters, which are important in the early stages of infection. This work investigated how host microenvironments, in particular acidic containing lactic acid, affect C. albicans biofilm formation and antifungal drug resistance. Multiple components of the extracellular matrix were also analysed, including their impact on antifungal drug resistance, and the involvement of both Jen1 and Jen2 in this process. The results show that growth on lactate affects biofilm formation, morphology and susceptibility to fluconazole and that both Jen1 and Jen2 might play a role in these processes. These results support the view that the adaptation of Candida cells to the carbon source present in the host niches affects their pathogenicity.
白色念珠菌有能力适应不同的宿主生态位，这些生态位通常葡萄糖含量有限，但富含其他碳源。在这些葡萄糖匮乏的微环境中，这种病原体表达JEN1和JEN2基因，这两个基因编码羧酸转运蛋白，在感染早期阶段很重要。这项研究调查了宿主微环境，特别是含乳酸的酸性微环境，如何影响白色念珠菌生物膜的形成和抗真菌药物耐药性。还分析了细胞外基质的多个成分，包括它们对抗真菌药物耐药性的影响，以及Jen1和Jen2在这一过程中的作用。结果表明，在乳酸上生长会影响生物膜的形成、形态以及对氟康唑的敏感性，并且Jen1和Jen2可能在这些过程中发挥作用。这些结果支持这样一种观点，即念珠菌细胞对宿主生态位中存在的碳源的适应会影响它们的致病性。
