Martina Pablo, Leguizamon Mariana, Prieto Claudia I, Sousa Silvia A, Montanaro Patricia, Draghi Walter O, Stämmler Maren, Bettiol Marisa, de Carvalho Carla C C R, Palau Juliana, Figoli Cecilia, Alvarez Florencia, Benetti Silvina, Lejona Sergio, Vescina Cecilia, Ferreras Julián, Lasch Peter, Lagares Antonio, Zorreguieta Angeles, Leitão Jorge H, Yantorno Osvaldo M, Bosch Alejandra
CINDEFI, CONICET-CCT La Plata, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
Present address: IBS, CONICET-CCT Nordeste, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Misiones, Argentina.
Int J Syst Evol Microbiol. 2018 Jan;68(1):14-20. doi: 10.1099/ijsem.0.002293. Epub 2017 Nov 2.
Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by more than 3 % from those of the Bcc with validly published names. According to the multilocus sequence analysis (MLSA), these isolates clustered with the agricultural soil strain, Burkholderia sp. PBP 78, which was already deposited in the PubMLST database. The isolates were examined using a polyphasic approach, which included 16S rRNA, recA, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA base composition, average nucleotide identities (ANIs), fatty acid profiles, and biochemical characterizations. The results of the present study demonstrate that the seven isolates represent a single novel species within the Bcc, for which the name Burkholderia puraquae sp. nov. is proposed. Burkholderia puraquae sp. nov. CAMPA 1040 (=LMG 29660=DSM 103137) was designated the type strain of the novel species, which can be differentiated from other species of the Bcc mainly from recA gene sequence analysis, MLSA, ANIb, MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 °C, aesculin hydrolysis, and lysine decarboxylase and β-galactosidase activities.
洋葱伯克霍尔德菌复合体（Bcc）中的细菌能够在囊性纤维化（CF）患者中引起严重感染。这些机会致病菌也广泛分布于自然和人造环境中。在对阿根廷CF患者呼吸道分泌物及医院环境中的Bcc细菌进行了12年的流行病学监测后，我们发现了6株Bcc分离株，其串联的种特异性等位基因序列与已有效发表名称的Bcc分离株相比差异超过3%。根据多位点序列分析（MLSA），这些分离株与已保藏于PubMLST数据库的农业土壤菌株伯克霍尔德菌属PBP 78聚类在一起。采用多相方法对这些分离株进行了检测，包括16S rRNA、recA、基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）、DNA碱基组成、平均核苷酸同一性（ANI）、脂肪酸谱和生化特性分析。本研究结果表明，这7株分离株代表了Bcc内的一个新物种，为此提出了伯克霍尔德菌新种（Burkholderia puraquae sp. nov.）的名称。伯克霍尔德菌新种（Burkholderia puraquae sp. nov.）CAMPA 1040（=LMG 29660=DSM 103137）被指定为该新物种的模式菌株，它主要可通过recA基因序列分析、MLSA、ANIb、MALDI-TOF MS分析以及一些生化试验，包括在42°C下生长的能力、七叶苷水解、赖氨酸脱羧酶和β-半乳糖苷酶活性，与Bcc的其他物种区分开来。
