Department of Clinical Analysis, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Av. do Café S/N. Monte Alegre, Ribeirão Preto, SP, 14040-903, Brazil.
School of Dentistry, University of Ribeirao Preto (UNAERP), Ribeirão Preto, Brazil.
World J Microbiol Biotechnol. 2019 Feb 9;35(3):39. doi: 10.1007/s11274-019-2614-0.
The correct identification of different genera and bacterial species is essential, especially when these bacteria cause infections and appropriate therapies need to be chosen. Bacteria belonging to the Burkholderia cepacia complex are considered important opportunistic pathogens, causing different types of infections in immunocompromised, principally in patients with cystic fibrosis. Twenty-one isolates were obtained from different soil samples and identified by sequencing of 16S rRNA, 23S rRNA, recA gene, MLST and by VITEK 2 and MALDI-TOF MS systems. Then, statistical analyses were performed. VITEK 2 and MALDI-TOF MS systems showed different bacterial genera. Sequencing of the 16S rRNA, 23S rRNA gene and amplification of recA gene showed that all the isolates belong to the B. cepacia complex. Sequencing of the recA gene showed a predominance of B. cenocepacia. The PCR of the recA gene showed a high specificity when it is necessary to identify the bacteria belonging to the B. cepacia complex in comparison with 16S and 23S rRNA genes sequencing. MLST analyzes showed a diversity of STs, which have not yet been correlated to the species. Phenotypic identification was not suitable for the identification of these pathogens since in many cases different genera have been reported, including identification by using MALDI-TOF MS.
正确识别不同属和细菌种是必要的，特别是当这些细菌引起感染时，需要选择适当的治疗方法。伯克霍尔德氏菌属复合群中的细菌被认为是重要的机会致病菌，会导致免疫功能低下的患者（主要是囊性纤维化患者）发生不同类型的感染。从不同土壤样本中获得了 21 个分离株，并通过 16S rRNA、23S rRNA、recA 基因、MLST 和 VITEK 2 和 MALDI-TOF MS 系统测序进行了鉴定。然后进行了统计分析。VITEK 2 和 MALDI-TOF MS 系统显示出不同的细菌属。16S rRNA、23S rRNA 基因测序和 recA 基因扩增表明，所有分离株均属于伯克霍尔德氏菌属复合群。recA 基因测序显示，以 B. cenocepacia 为主。与 16S 和 23S rRNA 基因测序相比，recA 基因的 PCR 显示出更高的特异性，当需要鉴定属于伯克霍尔德氏菌属复合群的细菌时。MLST 分析显示了 STs 的多样性，尚未与物种相关联。表型鉴定不适合这些病原体的鉴定，因为在许多情况下，包括使用 MALDI-TOF MS 鉴定，都会报告不同的属。
