Coco Marinella, Perciavalle Vincenzo, Cavallari Paolo, Bolzoni Francesco, Graziano Adriana Carol Eleonora, Perciavalle Valentina
Department of Biomedical and Biotechnological Sciences, Physiology Section Department of Pathophysiology and Transplantation, Human Physiology Section, University of Milan, Milan Department of Sciences of Formation, University of Catania, Catania, Italy.
Medicine (Baltimore). 2017 Nov;96(44):e8325. doi: 10.1097/MD.0000000000008325.
It has been observed that, after 2 hours of aerobic exercise, plasma interleukin-6 (IL-6) increases whereas nuclear concentrations of enzyme DNA methyltransferase (DNMT) 3B significantly decreased in peripheral blood mononuclear cells (PBMCs), with no change observed in DNMT3A. The aim of the present study was to detect differences in these changes induced by exercise in plasma IL-6 levels as well as in PBMC nuclear concentrations of DNMT3A and DNMT3B, in relation to age and sex. Four groups were studied: 12 young men (24.8 ± 1.77 years old), 12 young women (23.8 ± 1.81 years old), 12 adult men (45.8 ± 1.82 years old), 12 adult women (mean 44.5 ± 2.07 years old). Participants had to run at 60% of maximal oxygen consumption (VO2max) for 120 minutes, interspersed with sprints at 90% of VO2max for the last 30 seconds of every 10 minutes. About 250 μL of PBMCs (1 × 10 cells) were treated with 100 μL of either pre-exercise plasma or post-exercise plasma and nuclear DNMT3A and DNMT3B concentrations were quantified. No change in nuclear concentration of DNMT3A following the exercise was observed. Conversely, nuclear concentrations of DNMT3B significantly decreased, with a reduction of about 78% in young men, 72% in young women, 61% in adult men, and 53% in adult women. Moreover, a strong positive correlation between the nuclear concentration of DNMT3B in PBMC following stimulation with post-exercise plasma and post-exercise plasma concentrations of IL-6 was observed in all the 4 studied groups. This study confirms that a single bout of endurance exercise is sufficient to decrease nuclear concentrations of DNMT3B and thus protein upregulation. Moreover, the epigenetic mechanisms induced by exercise apparently cause more intense changes in men than in women and that, in both of them, this effect seems to decrease with age.
据观察，有氧运动2小时后，外周血单核细胞（PBMC）中血浆白细胞介素-6（IL-6）增加，而酶DNA甲基转移酶（DNMT）3B的核浓度显著降低，DNMT3A未观察到变化。本研究的目的是检测运动诱导的血浆IL-6水平以及PBMC中DNMT3A和DNMT3B的核浓度变化在年龄和性别方面的差异。研究了四组：12名年轻男性（24.8±1.77岁）、12名年轻女性（23.8±1.81岁）、12名成年男性（45.8±1.82岁）、12名成年女性（平均44.5±2.07岁）。参与者必须以最大耗氧量（VO2max）的60%跑步120分钟，每10分钟的最后30秒穿插以VO2max的90%进行冲刺。用100μL运动前血浆或运动后血浆处理约250μL PBMC（1×10个细胞），并对核DNMT3A和DNMT3B浓度进行定量。运动后未观察到DNMT3A核浓度的变化。相反，DNMT3B的核浓度显著降低，年轻男性降低约78%，年轻女性降低72%，成年男性降低61%，成年女性降低53%。此外，在所有4个研究组中，运动后血浆刺激后PBMC中DNMT3B的核浓度与运动后血浆IL-6浓度之间存在强正相关。本研究证实，单次耐力运动足以降低DNMT3B的核浓度，从而减少蛋白质上调。此外，运动诱导的表观遗传机制在男性中引起的变化显然比女性更强烈，并且在两者中，这种效应似乎随着年龄的增长而降低。
