Li Wen-Hwa, Fassih Ali, Binner Curt, Parsa Ramine, Southall Michael D
The Johnson & Johnson Skin Research Center, Johnson & Johnson Consumer Inc., Skillman, New Jersey.
Lasers Surg Med. 2018 Feb;50(2):158-165. doi: 10.1002/lsm.22747. Epub 2017 Nov 2.
Acne vulgaris is a chronic inflammatory disease of the pilosebaceous units (PSU), associated with increased sebum production, abnormal follicular keratinization (hyperkeratinization), follicular overgrowth of Propionibacterium acnes (P. acnes), and increased inflammatory mediator release. Light therapy has attracted medical interests as a safe alternative treatment for acne. Both blue and red light therapies at high doses >10 J/cm have demonstrated marked effects on inflammatory acne lesions. However, few studies have investigated the effects of lower doses of light. The aim of this study is to investigate the biological effects of lower doses of red light at 0.2-1.2 J/cm for acne using an in vitro model previously developed to mimic the inflammation and hyperkeratinization observed clinically in acne.
Human epidermal equivalents were topically exposed to an unsaturated fatty acid, oleic acid (OA), followed by red light-emitting diode (LED) light treatments (light-plus-OA treatments). Endpoints evaluated included the proinflammatory cytokine IL-1α, epidermal barrier integrity, as measured by transepithelial electrical resistance (TEER), and stratum corneum (SC) thickness to monitor hyperkeratinization.
OA-induced IL-1α release was significantly (P < 0.05) reduced following red LED light at 0.2, 0.5, and 1.2 J/cm , from 266 ± 11 pg/ml of no-light-plus-OA-treated (OA treatment without light) controls to 216 ± 9, 231 ± 8, and 212 ± 7 pg/ml, respectively. Histological examination showed that SC thickening following OA treatment was reduced from 43% of total epidermis for no-light-plus-OA treatment to 37% and 38% of total epidermis following 0.5 and 1.1 J/cm red light plus OA treatment, respectively (P < 0.05). Moreover, 1.1 J/cm red-light-plus-OA treatment improved OA-induced TEER changes from 29% of baseline for no-light-plus-OA treatment, to 36% of baseline.
Low level red LED light therapy could provide beneficial effects of anti-inflammation, normalizing pilosebaceous hyperkeratinization, and improving barrier impairment in Acne vulgaris. Lasers Surg. Med. 50:158-165, 2018. © 2017 Wiley Periodicals, Inc.
寻常痤疮是一种毛囊皮脂腺单位（PSU）的慢性炎症性疾病，与皮脂分泌增加、毛囊角化异常（过度角化）、痤疮丙酸杆菌（P. acnes）的毛囊过度生长以及炎症介质释放增加有关。光疗作为一种安全的痤疮替代治疗方法已引起医学关注。高剂量（>10 J/cm）的蓝光和红光疗法对炎性痤疮皮损均显示出显著效果。然而，很少有研究调查较低剂量光的作用。本研究的目的是使用先前开发的体外模型来模拟临床上在痤疮中观察到的炎症和过度角化，研究0.2 - 1.2 J/cm较低剂量红光对痤疮的生物学效应。
将人表皮替代物局部暴露于不饱和脂肪酸油酸（OA），随后进行红色发光二极管（LED）光治疗（光加OA治疗）。评估的终点包括促炎细胞因子IL - 1α、通过跨上皮电阻（TEER）测量的表皮屏障完整性以及监测过度角化的角质层（SC）厚度。
在0.2、0.5和1.2 J/cm的红色LED光照射后，OA诱导的IL - 1α释放显著（P < 0.05）降低，从无光加OA治疗（无光的OA治疗）对照组的266 ± 11 pg/ml分别降至216 ± 9、231 ± 8和212 ± 7 pg/ml。组织学检查显示，OA治疗后的SC增厚从无光加OA治疗时占总表皮的43%分别降至0.5和1.1 J/cm红光加OA治疗后的37%和38%（P < 0.05）。此外，1.1 J/cm红光加OA治疗将OA诱导的TEER变化从无光加OA治疗时的基线的29%改善至基线的36%。
低水平红色LED光疗可对寻常痤疮产生抗炎、使毛囊皮脂腺过度角化正常化以及改善屏障损伤的有益作用。激光外科与医学。50:158 - 165，2018。© 2017威利期刊公司。
