黏液表皮样癌的 miRNA 表达谱。
miRNA expression profile of mucoepidermoid carcinoma.
机构信息
Department of Oral Diagnostic Sciences, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Oncology and Diagnostic Sciences, University of Maryland Dental School, Baltimore, MD, USA.
出版信息
Oral Dis. 2018 May;24(4):537-543. doi: 10.1111/odi.12800. Epub 2018 Feb 8.
OBJECTIVES
MicroRNAs (miRNAs) are single-stranded RNAs that have been implicated in cancer initiation and progression and act as tumour suppressors or oncogenes. In this study, miRNA profiling was conducted on the most frequent malignancy of salivary glands, mucoepidermoid carcinoma (MEC), in comparison with normal tissues.
MATERIALS AND METHODS
The TaqMan Human miRNA Cards Array was used for the miRNA profiling of MEC and normal tissues. To validate the differentially expressed miRNAs in MEC, we used real-time PCR (qRT-PCR).
RESULTS
miR-302a was the most significantly increased miRNA in cancer tissues (p < .05). Here, we demonstrate that the upregulation of miR-302a expression in SGT cell lines induced cancer cell invasion in vitro.
CONCLUSIONS
These promising results suggest the need for further studies to establish mir-302a as a marker of invasion and aggressiveness in MEC.
目的
微小 RNA（miRNA）是单链 RNA，已被认为与癌症的发生和进展有关，并作为肿瘤抑制因子或癌基因发挥作用。在这项研究中，与正常组织相比，对唾液腺最常见的恶性肿瘤——黏液表皮样癌（MEC）进行了 miRNA 谱分析。
材料和方法
使用 TaqMan 人类 miRNA 卡式阵列对 MEC 和正常组织进行 miRNA 谱分析。为了验证 MEC 中差异表达的 miRNA，我们使用了实时 PCR（qRT-PCR）。
结果
miR-302a 在癌症组织中表达显著增加（p<.05）。在这里，我们证明 SGT 细胞系中 miR-302a 表达的上调诱导了体外癌细胞侵袭。
结论
这些有前途的结果表明，需要进一步研究，以确定 mir-302a 作为 MEC 侵袭和侵袭性的标志物。
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