Department of Pathology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Republic of Korea.
Department of Pathology, Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Republic of Korea.
Sci Rep. 2022 Aug 4;12(1):13383. doi: 10.1038/s41598-022-17740-9.
Carcinoma ex pleomorphic adenoma (CXPA) is a rare malignancy that transforms from PA. Early detection of the carcinoma by biopsy is difficult due to similar histopathology of the malignant and benign components. To address this, we investigated and compared the characteristic miRNA expression patterns across samples of the PA, carcinomatous portions (CA) of CXPA, as well as conventional PA. We selected 13 CXPA and 16 conventional PA FFPE samples, separated the PA and CA portions of CXPA samples and conducted miRNA profiling for each group. Among 13 transcripts that were differentially expressed between PA and CA of CXPA, eight miRNAs were up-regulated and five down-regulated in CA. Bioinformatic analysis revealed that the up-regulated miRNAs were related to cancer progression and down-regulated ones to tumor suppression. Additionally, seven miRNAs were significantly up-regulated in PA of CXPA compared to conventional PA, although they are histopathologically similar. Almost all of these transcripts interacted with TP53, a well-known tumor suppressor. In conclusion, we identified differentially expressed miRNAs in PA and CA of CXPA, which were closely associated with TP53 and various cancer-related pathways. We also identified differentially expressed miRNAs in the PA of CXPA and conventional PA which may serve as potential biomarkers.
癌在多形性腺瘤中（CXPA）是一种罕见的恶性肿瘤，由 PA 转化而来。由于恶性和良性成分的组织病理学相似，通过活检早期发现癌是困难的。为了解决这个问题，我们研究并比较了 PA、CXPA 的癌部分（CA）以及常规 PA 的特征性 miRNA 表达模式。我们选择了 13 个 CXPA 和 16 个常规 PA FFPE 样本，分离 CXPA 样本的 PA 和 CA 部分，并对每个组进行 miRNA 分析。在 PA 和 CA 之间差异表达的 13 个转录本中，8 个 miRNA 在 CA 中上调，5 个下调。生物信息学分析显示，上调的 miRNA 与癌症进展有关，下调的 miRNA 与肿瘤抑制有关。此外，与常规 PA 相比，CXPA 的 PA 中有 7 个 miRNA 显著上调，尽管它们在组织病理学上相似。这些转录物几乎都与 TP53 相互作用，TP53 是一种众所周知的肿瘤抑制因子。总之，我们鉴定了 CXPA 的 PA 和 CA 中差异表达的 miRNA，它们与 TP53 和各种癌症相关途径密切相关。我们还鉴定了 CXPA 的 PA 和常规 PA 中差异表达的 miRNA，它们可能作为潜在的生物标志物。
