Liu Hai-Bin, Du Xin-Jun, Zang Yu-Xuan, Li Ping, Wang Shuo
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology , Tianjin 300457, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU) , Beijing 100048, China.
J Agric Food Chem. 2017 Nov 29;65(47):10290-10299. doi: 10.1021/acs.jafc.7b03957. Epub 2017 Nov 14.
Rapid, sensitive, point-of-care detection of bacteria is extremely important in food safety. To address this requirement, we developed a new surface-enhanced Raman scattering (SERS)-based lateral flow (LF) strip biosensor combined with recombinase polymerase amplification (RPA) for simultaneous detection of Listeria monocytogenes and Salmonella enterica serotype Enteritidis. Au@Ag core-shell nanoparticles were used in this SERS-LF. Highly sensitive quantitative detection is achieved by measuring the characteristic peak intensities of SERS tags. Under optimal conditions, the SERS intensities of MBA at 1077 cm on test lines are used to measure S. Enteritidis (y = 1980.6x - 539.3, R = 0.9834) and L. monocytogenes (y = 1696.0x - 844, R = 0.9889), respectively. The limit of detection is 27 CFU/mL for S. Enteritidis and 19 CFU/mL for L. monocytogenes. Significantly, this SERS-LF has high specificity and applicability in the detection of L. monocytogenes and S. Enteritidis in food samples. Therefore, the SERS-LF is a feasible method for the rapid and quantitative detection of a broad range of bacterial pathogens in real food samples.
快速、灵敏的即时检测细菌在食品安全中极为重要。为满足这一需求，我们开发了一种基于表面增强拉曼散射（SERS）的新型侧向流动（LF）条带生物传感器，并结合重组酶聚合酶扩增（RPA）技术，用于同时检测单核细胞增生李斯特菌和肠炎沙门氏菌肠炎血清型。此SERS-LF中使用了金@银核壳纳米颗粒。通过测量SERS标签的特征峰强度实现高灵敏定量检测。在最佳条件下，测试线上1077 cm处MBA的SERS强度分别用于检测肠炎沙门氏菌（y = 1980.6x - 539.3，R = 0.9834）和单核细胞增生李斯特菌（y = 1696.0x - 844，R = 0.9889）。肠炎沙门氏菌的检测限为27 CFU/mL，单核细胞增生李斯特菌的检测限为19 CFU/mL。值得注意的是，这种SERS-LF在食品样品中检测单核细胞增生李斯特菌和肠炎沙门氏菌时具有高特异性和适用性。因此，SERS-LF是一种在实际食品样品中快速定量检测多种细菌病原体的可行方法。
