Bashkir Scientific Research Institute of Agriculture, Russian Academy of Sciences, R. Zorge St.19, 450059 Ufa, Russia.
Bashkir Scientific Research Institute of Agriculture, Russian Academy of Sciences, R. Zorge St.19, 450059 Ufa, Russia.
Plant Physiol Biochem. 2017 Dec;121:80-88. doi: 10.1016/j.plaphy.2017.10.020. Epub 2017 Nov 6.
Endophytic strain Bacillus subtilis (B. subtilis) 10-4, producing indole-3-acetic acid (IAA) and siderofores but not active in phosphate solubilization, exerted a protective effect on Triticum aestivum L. (wheat) plant grown under salinity (2% NaCl) stress. Exposure to salt stress resulted in an essential increase of proline (Pro) and malondialdehyde (MDA) level in the seedlings. At the same time the seedlings inoculated with B. subtilis 10-4 were characterized by decreased level of stress-induced Pro and MDA accumulation. It was revealed that both B. subtilis 10-4 and salinity caused increase in the content of endogenous salicylic acid (SA) in wheat seedlings as compared to SA content in the control, while B. subtilis 10-4 suppressed stress-induced SA accumulation. Water storage capacity (WSC) in leaf tissues was increased and stress-induced hydrolysis of statolite starch in root cap cells of the germinal roots was reduced by B. subtilis 10-4. The obtained data indicated that the activation of the defense reactions induced by B. subtilis 10-4 induced defense reactions may be connected with their ability to decrease the level of stress-induced oxidative and osmotic stress in seedlings and with the increase of endogenous SA level that can make a significant contribution to the implementation of the protective effect of B. subtilis 10-4 and is manifested in the improvement of plant growth, WSC of leaves and slowing down of the process of statolite starch hydrolysis under salinity.
内生枯草芽孢杆菌（B. subtilis）10-4 菌株产生吲哚-3-乙酸（IAA）和铁载体，但不具有磷酸盐溶解活性，对在盐胁迫（2% NaCl）下生长的小麦（Triticum aestivum L.）植株具有保护作用。暴露于盐胁迫会导致幼苗中脯氨酸（Pro）和丙二醛（MDA）水平显著增加。同时，用枯草芽孢杆菌 10-4 接种的幼苗表现出应激诱导的 Pro 和 MDA 积累减少。结果表明，枯草芽孢杆菌 10-4 和盐胁迫都会导致小麦幼苗中内源性水杨酸（SA）含量增加，与对照相比，而枯草芽孢杆菌 10-4 抑制了应激诱导的 SA 积累。叶片组织的水分储存能力（WSC）增加，根冠细胞中胚根的 statolite 淀粉的应激诱导水解减少。所得数据表明，枯草芽孢杆菌 10-4 诱导的防御反应的激活可能与其降低幼苗中应激诱导的氧化和渗透胁迫水平的能力有关，并且与内源性 SA 水平的增加有关，这对枯草芽孢杆菌 10-4 的保护作用的实施有重大贡献，表现在改善植物生长、叶片水分储存能力和减缓盐胁迫下 statolite 淀粉水解的过程。
