Department of Physical, Earth and Environmental Sciences, University of Siena, Italy.
Department of Physical, Earth and Environmental Sciences, University of Siena, Italy.
Ecotoxicol Environ Saf. 2018 Feb;148:377-383. doi: 10.1016/j.ecoenv.2017.10.050. Epub 2017 Nov 6.
The uptake of Cd and some biomarkers of exposure and effects have been investigated in specimens of land snail Cornu aspersum exposed to vaporized CdCl (10mg/L) for 7 days. The Cd levels quantified in snail's whole bodies confirmed Cd bioavailability trough vaporization and an higher accumulation in the midgut gland compared to the foot. Biological responses investigated showed a reduction of destabilization time of lysosomal membranes (NRRT) in hemocytes and an induction of catalase activities (CAT) in midgut gland. A further evidence of CdCl vaporized exposure was given by an increase in MT protein content as well as induction of Cd-MT gene expression, highlighting the central role of the midgut gland in Cd detoxification. These biomarkers can thus be considered as sensitive tools for the assessment of Cd contamination in the air using land snails as bioindicators. No changes in of GST activity and MDA were observed. From the overall results, the land snail, C. aspersum, could be used as good bioindicator of air quality for pollution monitoring purposes having shown clear signs of exposure and effects due Cd exposure by air.
已经研究了在暴露于蒸发的 CdCl（10mg/L）7 天后的陆生蜗牛 Cornu aspersum 标本中，Cd 的摄取以及一些暴露和效应的生物标志物。蜗牛全身定量的 Cd 水平证实了通过蒸发使 Cd 具有生物利用度，并且与足部相比，在中肠腺中的积累更高。研究的生物学反应表明，血细胞中的溶酶体膜（NRRT）的不稳定性时间减少，并且中肠腺中的过氧化氢酶活性（CAT）诱导。通过 MT 蛋白含量的增加以及 Cd-MT 基因表达的诱导，进一步证明了 CdCl 蒸发暴露，突出了中肠腺在 Cd 解毒中的核心作用。因此，这些生物标志物可以被认为是使用陆生蜗牛作为生物指示剂评估空气中 Cd 污染的敏感工具。未观察到 GST 活性和 MDA 的变化。从整体结果来看，陆生蜗牛 Cornu aspersum 可以作为空气质量的良好生物指示剂，用于污染监测目的，因为它显示出由于空气中 Cd 暴露而导致的暴露和效应的明显迹象。
