Cybularz Maria, Langbein Heike, Zatschler Birgit, Brunssen Coy, Deussen Andreas, Matschke Klaus, Morawietz Henning
Division of Vascular Endothelium and Microcirculation, Department of Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, Germany.
Institute of Physiology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstr. 74, 01307 Dresden, Germany.
Atheroscler Suppl. 2017 Nov;30:149-158. doi: 10.1016/j.atherosclerosissup.2017.05.042. Epub 2017 Jun 3.
Obesity is a risk factor for endothelial dysfunction and atherosclerosis. However, perivascular adipose tissue can release adipokines and other unknown adipose-derived relaxing factors. Therefore, we investigated the impact of obesity on vascular function and expression of genes in perivascular adipose tissue from internal mammary arteries of patients with coronary artery disease undergoing coronary artery bypass grafting.
The vessel function was compared between groups of patients with a body-mass index (BMI) between 25 and 30 kg/m. The groups did not differ in age, gender (males), and ejection fraction. Vascular segments of internal mammary arteries were examined in a Mulvany myograph. Following preconstriction with noradrenaline, dose-response curves were assessed for relaxation with acetylcholine and sodium nitroprusside.
Maximum contraction in response to potassium and noradrenaline was increased in obese patients with a BMI >30 kg/m. EC50 of endothelium-dependent relaxation was impaired in patients with a BMI above 25, but below 30 kg/m. Sodium nitroprusside-mediated maximal relaxation was not different between study groups. Integrin alpha X chain (ITGAX/CD11c) and macrophage mannose receptor (MRC1/CD206) expression was reduced in perivascular adipose tissue of patients with a BMI above 30 kg/m, while adiponectin (ADPQ) expression was increased in the same tissue.
Our data suggest a partially reduced endothelial function in internal mammary arteries of adipose patients with a BMI between 25 and 30 kg/m undergoing coronary artery bypass grafting surgery. Increased adiponectin expression in perivascular tissue might contribute to maintenance of endothelial function in obese patients with a BMI above 30 kg/m.
肥胖是内皮功能障碍和动脉粥样硬化的危险因素。然而，血管周围脂肪组织可释放脂肪因子和其他未知的脂肪源性舒张因子。因此，我们研究了肥胖对接受冠状动脉搭桥术的冠心病患者乳内动脉血管周围脂肪组织血管功能和基因表达的影响。
比较体重指数（BMI）在25至30kg/m²之间的患者组之间的血管功能。这些组在年龄、性别（男性）和射血分数方面没有差异。在Mulvany肌动描记器中检查乳内动脉的血管段。用去甲肾上腺素预收缩后，评估乙酰胆碱和硝普钠舒张的剂量反应曲线。
BMI>30kg/m²的肥胖患者对钾和去甲肾上腺素的最大收缩增加。BMI高于25但低于30kg/m²的患者内皮依赖性舒张的半数有效浓度（EC50）受损。硝普钠介导的最大舒张在研究组之间没有差异。BMI高于30kg/m²的患者血管周围脂肪组织中整合素αX链（ITGAX/CD11c）和巨噬细胞甘露糖受体（MRC1/CD206）表达降低，而同一组织中脂联素（ADPQ）表达增加。
我们的数据表明，BMI在25至30kg/m²之间接受冠状动脉搭桥手术的肥胖患者乳内动脉的内皮功能部分降低。血管周围组织中脂联素表达增加可能有助于BMI高于30kg/m²的肥胖患者维持内皮功能。
