Gruzdeva Olga V, Dyleva Yulia A, Belik Ekaterina V, Sinitsky Maxim Y, Stasev Aleksandr N, Kokov Aleksandr N, Brel Natalia K, Krivkina Evgenia O, Bychkova Evgenia E, Tarasov Roman S, Barbarash Olga L
Federal State Budgetary Scientific Institution "Research Institute for Complex Issues of Cardiovascular Diseases", 650002 Kemerovo, Russia.
J Pers Med. 2022 Jan 19;12(2):129. doi: 10.3390/jpm12020129.
Adipose tissue (AT) is an endocrine and paracrine organ that synthesizes biologically active adipocytokines, which affect inflammation, fibrosis, and atherogenesis. Epicardial and perivascular fat depots are of great interest to researchers, owing to their potential effects on the myocardium and blood vessels. The aim of the study was to assess the expression and secretion of adipocytokine genes in the AT of patients with coronary artery disease (CAD) and patients with aortic or mitral valve replacement. This study included 84 patients with CAD and 50 patients with aortic or mitral valve replacement. Adipocytes were isolated from subcutaneous, epicardial (EAT), and perivascular AT (PVAT), and were cultured for 24 h. EAT exhibited the lowest level of adiponectin gene expression and secretion, regardless of nosology, and high expression levels of the leptin gene and interleukin-6 (IL-6). However, EAT adipocytes in patients with CAD were characterized by more pronounced changes in comparison with the group with heart defects. High leptin and IL-6 levels resulted in increased pro-inflammatory activity, as observed in both EAT and PVAT adipocytes, especially in individuals with CAD. Therefore, our results revealed the pathogenetic significance of alterations in the adipokine and cytokine status of adipocytes of EAT and PVAT in patients with CAD.
脂肪组织（AT）是一个内分泌和旁分泌器官，可合成具有生物活性的脂肪细胞因子，这些因子会影响炎症、纤维化和动脉粥样硬化的发生。由于心外膜和血管周围脂肪库对心肌和血管具有潜在影响，因此受到研究人员的极大关注。本研究的目的是评估冠心病（CAD）患者以及接受主动脉或二尖瓣置换术患者的脂肪组织中脂肪细胞因子基因的表达和分泌情况。本研究纳入了84例CAD患者和50例接受主动脉或二尖瓣置换术的患者。从皮下、心外膜（EAT）和血管周围脂肪组织（PVAT）中分离脂肪细胞，并培养24小时。无论疾病分类如何，EAT中脂联素基因的表达和分泌水平最低，而瘦素基因和白细胞介素-6（IL-6）的表达水平较高。然而，与心脏缺陷组相比，CAD患者的EAT脂肪细胞表现出更明显的变化。在EAT和PVAT脂肪细胞中均观察到，高瘦素和IL-6水平导致促炎活性增加，尤其是在CAD患者中。因此，我们的研究结果揭示了CAD患者EAT和PVAT脂肪细胞中脂肪因子和细胞因子状态改变的发病机制意义。
