Drug Radiation Research Department, Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Egypt.
Drug Radiation Research Department, Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Egypt.
Int J Biol Macromol. 2018 Feb;107(Pt B):2298-2311. doi: 10.1016/j.ijbiomac.2017.10.121. Epub 2017 Oct 31.
Aqueous dispersed zinc nanoparticles (ZnNPs) were synthesized using natural polysaccherides (chitosan (CS), citrus pectin (CP) and alginate (Alg)) using aqueous fermented fenugreek powder (FFP) by Pleurotus ostreatus as reducing and stabilizing agent or using gamma irradiation. The synthesized ZnNPs are characterized by ultra violect spectroscopy (UV), Transmission electronmicroscopy (TEM), Dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). XRD analysis of the ZnNPS confirmed the formation of metallic nanoparticles. The nucleation and growth mechanism of ZnNPs is also discussed. TEM showed that the average diameter of ZnNPs was in the range of 46nm. The size of nanoparticles is influenced by certain parameters such as the choice of stabilizer, pH during synthesis and absorbed dose. Evaluating the antioxidant and anticancer activities of ZnNPs was performed. The results indicating the ZnNPs synthesized by aqueous fermented fenugreek extract have high activity and the average size is 46nm. The results explored that ZnNPs show anticancer activity against Ehrlich Ascites Carcinoma (EAC) and human colon adenocarcinoma (CACO) and the IC was 47.5μg/ml and 65μg/ml respectively. Also, ZnNPs had excellent bactericidal activity against gram positive and negative bacteria and yeast.
水相分散的锌纳米粒子（ZnNPs）是通过使用天然多糖（壳聚糖（CS）、柑橘果胶（CP）和藻酸盐（Alg））和用水发酵的葫芦巴粉（FFP）由榆黄蘑（Pleurotus ostreatus）作为还原剂和稳定剂，或使用伽马射线合成的。所合成的 ZnNPs 通过紫外分光光度法（UV）、透射电子显微镜（TEM）、动态光散射（DLS）、X 射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）进行表征。ZnNPS 的 XRD 分析证实了金属纳米粒子的形成。还讨论了 ZnNPs 的成核和生长机制。TEM 显示 ZnNPs 的平均直径在 46nm 范围内。纳米粒子的尺寸受某些参数的影响，例如稳定剂的选择、合成过程中的 pH 值和吸收剂量。评估了 ZnNPs 的抗氧化和抗癌活性。结果表明，用水发酵的葫芦巴提取物合成的 ZnNPs 具有高活性，平均粒径为 46nm。结果表明，ZnNPs 对艾氏腹水癌（EAC）和人结肠腺癌（CACO）具有抗癌活性，IC 分别为 47.5μg/ml 和 65μg/ml。此外，ZnNPs 对革兰氏阳性和阴性细菌以及酵母具有优异的杀菌活性。
