Drug Microbiology Lab., Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Biometals. 2021 Aug;34(4):815-829. doi: 10.1007/s10534-021-00309-w. Epub 2021 Apr 24.
Aqueous glutathione selenium nano-incorporation (GSH-SeN-Inco) was prepared by gamma radiation in presence of microbial glutathione (GSH) and selenium dioxide. The novel prepared GSH-SeN-Inco are validated by UV-vis spectroscopy, TEM (17.5 nm), DLS, XRD, EDX and FTIR spectrum reveals the presence of GSH moiety that coating the selenium nanoparticles (SeNPs) forming GSH-SeN-Inco. The XRD analysis verified the presence of metallic SeNPs. The nucleation and radiolysis mechanism of GSH-SeN-Inco formation are also discussed. The size GSH-SeN-Inco (17.5 nm) is affected by certain factors such as concentration of GSH, selenium dioxide, and absorbed dose of gamma radiation. The present study explored the positive role of GSH-SeN-Inco as an antitumor activity against HepG-2 and MCF-7, with IC at a concentration of 1.00 and 0.9 mM, respectively. The GSH-SeN-Inco show significant scavenging activity at 33%. The GSH-SeN-Inco shows antimicrobial potential against Gram-negative and Gram-positive bacteria with significant MIC especially Escherichia coli ATCC 25922 at 5.20 µg/ml.
水相谷胱甘肽硒纳米共嵌入物（GSH-SeN-Inco）是通过γ辐射在微生物谷胱甘肽（GSH）和二氧化硒存在下制备的。通过紫外可见光谱、TEM（17.5nm）、DLS、XRD、EDX 和 FTIR 光谱对新型制备的 GSH-SeN-Inco 进行了验证，结果表明 GSH 部分存在于硒纳米颗粒（SeNPs）表面，形成 GSH-SeN-Inco。XRD 分析证实了金属 SeNPs 的存在。还讨论了 GSH-SeN-Inco 形成的成核和辐解机制。GSH-SeN-Inco（17.5nm）的尺寸受 GSH、二氧化硒和γ辐射吸收剂量等因素的影响。本研究探讨了 GSH-SeN-Inco 作为抗肿瘤活性物质对 HepG-2 和 MCF-7 的积极作用，其 IC 浓度分别为 1.00 和 0.9mM。GSH-SeN-Inco 在 33%时表现出显著的清除活性。GSH-SeN-Inco 对革兰氏阴性和革兰氏阳性细菌具有抗菌潜力，尤其是大肠杆菌 ATCC 25922，其 MIC 值为 5.20µg/ml。
