Nakayama M, Nakano S, Koga T, Niho Y
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Natl Cancer Inst. 1989 Jan 18;81(2):153-7. doi: 10.1093/jnci/81.2.153.
We investigated whether lethally irradiated, contact-sensitive Balb/3T3 cell confluent monolayers, termed "cell mats," could be used for the screening of human tumors for sensitivity to anticancer drugs. Cell mats greatly inhibited the growth of normal Balb/3T3 cells and normal human fibroblasts but did not inhibit the growth of human tumor cells. To assess chemosensitivity of tumor cells, we measured the [14C]thymidine (dThd) incorporation by tumor cells that had been plated and then treated with various anticancer drugs on the cell mats. Although the [14C]dThd incorporation assay, when compared with the colony-formation assay, underestimated the toxicity of certain drugs, the assays gave similar results with regard to the degree of colony inhibition and to the decrease in [14C]dThd incorporation when various anticancer drugs were used at the same concentration ranges. Therefore, the method we used for measuring the isotope uptake in the cell mats could also be used to test for tumor-specific chemosensitivity. Because our results from this assay on various human tumor cells, including those from primary human tumors, can be obtained within 5 days, we believe that the system is potentially useful for testing anticancer drugs against human tumors in vitro.
我们研究了经致死剂量照射的、具有接触敏感性的Balb/3T3细胞汇合单层，即所谓的“细胞垫”，是否可用于筛选人类肿瘤对抗癌药物的敏感性。细胞垫极大地抑制了正常Balb/3T3细胞和正常人成纤维细胞的生长，但不抑制人类肿瘤细胞的生长。为了评估肿瘤细胞的化学敏感性，我们测量了接种在细胞垫上然后用各种抗癌药物处理的肿瘤细胞对[14C]胸腺嘧啶核苷（dThd）的掺入情况。尽管与集落形成试验相比，[14C]dThd掺入试验低估了某些药物的毒性，但当在相同浓度范围内使用各种抗癌药物时，这两种试验在集落抑制程度和[14C]dThd掺入减少方面给出了相似的结果。因此，我们用于测量细胞垫中同位素摄取的方法也可用于检测肿瘤特异性化学敏感性。由于我们对各种人类肿瘤细胞（包括原发性人类肿瘤细胞）的该试验结果可在5天内获得，我们认为该系统在体外测试抗癌药物对人类肿瘤的作用方面可能具有潜在用途。
